子宫内膜异位症sICAM_医学论文.docVIP

子宫内膜异位症sICAM_医学论文 子宫内膜异位症sICAM_医学论文 【关键词】 子宫内膜异位症 Changes of sICAM1 and sHLAⅠin endometriosis and its significance 　　【Abstract】 AIM: To investigate the relationship between endometriosis (EMs) and the levels of soluble intercellular adhesion molecule1 (sICAM1) and soluble human leucocyte antigen1 (sHLAⅠ). METHODS: Thirty serum and 30 peritoneal fluid (PF) of patients with EMs (test group) and 30 serum and 30 peritoneal fluid of patients without EMs (control group) were studied. The levels of sICAM1 and sHLAⅠwere detected by enzymelinked immunosorbent assay (ELISA). RESULTS: ① Significantly higher levels of sICAM1 in serum and PF were observed in patients with EMs compared with those in controls (P0.05). ② The levels of sICAM1 in serum and PF were significantly different and the levels in earlystage EMs group were higher than those in advanced group (P0.05). ③ Serum sHLAⅠ cevel was lower in EMs group (P0.05) but the level in earlystage EMs group was higher than that in advanced group (P0.05). ④ There were no statistically significant differences in the levels of sHLAⅠin PF between the patients and the controls (P0.05). CONCLUSION: Changes of sICAM1and sHLAⅠmay play a role in the genesis of endometriosis. 　　【Keywords】 endometriosis HLA antigens intercellular adhesion molecule1 enzymelinked immunosorbent assay 　　【摘要】 目的： 探讨盆腔子宫内膜异位症（内异症）患者血清和腹腔液中可溶性细胞间粘附分子1(sICAM1)、可溶性人类白细胞抗原Ⅰ(sHLAⅠ)的水平与该病的发病关系. 方法： 以ELISA法定量检测sICAM1的水平、半定量检测sHLAⅠ水平. 结果： 内异症组血清和腹腔液中sICAM1和sHLAⅠ水平明显高于对照组(0.05). 结论： sICAM1，sHLAⅠ的变化可能与盆腔内异症发病有关，对sICAM1和sHLAⅠ的研究有利于了解内异症的发病机制和分期.　　【关键词】 子宫内膜异位症；HLA抗原；胞间粘附分子1；酶联免疫吸附测定 　　0引言　　子宫内膜异位症(内异症)是育龄妇女的常见妇科病,至今病因尚不十分清楚. 目前免疫机制被认为在其发病中起重要作用. 部分学者观察到，内异症患者ICAM1, HLAⅠ表达异常［1,2］. 本研究采用酶联免疫吸附法(ELISA)检测内异症患者血清和腹腔液中sICAM1, sHLAⅠ的水平，并探讨它们与疾病发生的关系. 　　1对象和方法　　1.1对象 　　选取200409/200502我科行手术治疗的内异症患者30例，年龄25～48（39±9）岁. 按RAFS标准，早期（Ⅰ～Ⅱ期）14例,晚期（Ⅲ～Ⅳ期）16例. 对照组为同期就诊的卵巢良性上皮性肿瘤患者30例，年龄22～44（36±7）岁. 实验组和对照组均经术后病检证实,月经周期正常,术前3 mo未使用激素类药物，无盆腔炎症,无自身免疫异常,无其他内、外科疾病，两组年龄无差异，均为汉族,来自湖北省. 　　1.2方法