子宫内膜样腺癌组织MMP9和CD147表达及意义_医学论文.docVIP

子宫内膜样腺癌组织MMP9和CD147表达及意义_医学论文 子宫内膜样腺癌组织MMP9和CD147表达及意义_医学论文 作者：公莉 戴淑真 田甜 王宁 车艳辞 【摘要】 　　目的 探讨基质金属蛋白酶（MMP）和细胞外基质金属蛋白酶诱导因子（CD147）在子宫内膜样腺癌组织表达及其与临床病理参数之间的关系。方法 采用逆转录聚合酶链反应（RT）技术检测40例子宫内膜样腺癌和12例正常子宫内膜组织中MMP和CD147基因的表达水平，并分析其与子宫内膜样腺癌临床分期、组织学分级、肌层浸润深度及淋巴结转移等病理参数的关系。结果 子宫内膜样腺癌组织中MMP和CD147的表达均明显高于正常子宫内膜（t=9.46、7.86,0.05）。MMP在子宫内膜样腺癌中的表达水平与其临床分期、组织学分级、肌层浸润深度及淋巴结转移有关（t=3.27～16.20，0.05）；CD147在子宫内膜样腺癌中的表达水平与其临床分期、组织学分级及淋巴结转移有关（t=2.50～6.20,0.05），与肌层浸润深度无关（t=1.91,Pgt0.05）。子宫内膜样腺癌中CD147和MMP的表达呈正相关（r=0.443,0.01）。结论 MMP和CD147的高表达可能在子宫内膜样腺癌的发生发展中起重要作用，二者相互协同可能是促进子宫内膜样腺癌发生、浸润和转移的重要因素。 【关键词】 子宫内膜肿瘤 基质金属蛋白酶 9 逆转录聚合酶链反应 　　［ABSTRACT］ Objective To investigate the expression of matrix metalloproteinasecarcinoma and its relationship with clinical pathology. Methods Reverse transcriptionpolymerase chain reaction (RTPCR) was used to detect MMP9 and CD147 in endometrial tissue of 40 patients with the carcinoma and 12 normal endometrial tissue. The relationship between the expressions and clinical pathology was analyzed.Results The expression of MMP9 and CD147 in the cancer was significantly higher than that of the normal tissue (t=9.46,7.860.05). Expression levels of both MMP9 and CD147 in the cancer were closely related to the clinical phases, histological grade, and lymph node metastasis (t=3.27-16.20,0.05t=2.50-6.20,0.05) the difference between them was that CD147 was not related to myometrial invasion. A positive correlation between the expression levels of MMP9 and CD147 in the cancer was noted (r=0.443,0.01). Conclusion High expression of MMP9 and CD147 may play an important role in the pathogenesis of endometrioid adenocarcinoma and their synergistic effect may act as important factors in promoting the invasion and metastasis of this cancer. 　　［KEY WORDS］ Endometrial neoplasms Matrix metalloproteinase9 Reverse transcriptionpolymerase chain reaction 　　子宫内膜癌是女性生殖系统常见的三大恶性肿瘤之一，约占妇科肿瘤的20%～30%，近年来发病率和病死率均有上升趋势[1,2]，其侵袭和转移是造成子宫内膜癌病人死亡的主要原因。多项研究发现，基质金属蛋白酶（MMPs）对细胞外基质（ECM）和基底膜（B