子宫内膜异位症和子宫腺肌病P450arom和COX2mRNA的表达及意义_医学论文.docVIP

子宫内膜异位症和子宫腺肌病P450arom和COX的表达及意义_医学论文 子宫内膜异位症和子宫腺肌病P450arom和COX的表达及意义_医学论文 【摘要】 目的 研究细胞色素芳香化酶P450（P450arom）基因和环氧化酶（COX）基因在子宫内膜异位症（EMs）和子宫腺肌病（AM）在位和异位内膜中的表达及相关性。方法 应用逆转录聚合酶链反应（RT）技术，检测22例正常子宫内膜、42例EMs及33例AM的在位和异位内膜中P450arom mRNA和COX的表达，并进行相关性分析。结果 P450arom mRNA在正常子宫内膜无表达，在EMs和AM的在位和异位内膜中均有表达，与正常子宫内膜比较差异均有显著性（F=28.777，q=4.624～10.282，P＜0.01)。P450arom mRNA在EMs和AM异位内膜中的表达均高于在位内膜，差异有显著性（q＝2.964、2.655，P＜0.01、0.05)。COX在正常子宫内膜无表达或弱表达，在EMs和AM的在位和异位内膜中均有表达，与正常子宫内膜比较差异均有显著性（F=21.307，q=2.583～7.905，P＜0.01)；在正常子宫内膜、EMs在位和异位子宫内膜中分泌期表达高于增殖期，差异均有显著性（t=4.401～15.865,P＜0.05)；在AM表达不随月经周期变化。COX在EMs和AM异位内膜中的表达均高于在位内膜，差异均有显著性（q=2.623、5.908，P＜0.05）。P450arom mRNA和COX在EMs的表达与rAFS分期均无相关性。P450arom mRNA与COX在EMs中的表达水平呈正相关（r=0.964，P＜0.01）；在AM中的表达水平亦呈正相关（r=0.813，P＜0.01）。结论 P450arom和COX与EMs和AM的发病相关，二者协同作用促进EMs和AM的发生发展。 【关键词】 芳香酶 环氧化酶2 子宫内膜异位症 子宫腺肌病 ［ABSTRACT］ObjectiveTo study the mRNA expressions of aromatase cytochrome P450 and cyclooxygenaseseases. MethodsThe expression of P450arom mRNA and COX2 mRNA were assessed by RTPCR in 22 cases of normal endometrium, 42 of EMs and 33 of AM including the eutopic and ectopic endometrium. The correlation between the mRNA expressions were analyzed. ResultsP450arom mRNA was expressed both in eutopic and ectopic endometrium of EMs and AM. But in normal endometrium, P450arom mRNA expression was not found. The difference between them was significant (F=28.777, q=4.624-10.282，P＜0.01). The P450arom mRNA expressions in ectopic endometrium of EMs and AM were significantly higher than that in eutopic endometrium (q＝2.964,2.655；P＜0.01, 0.05). COXficantly upregulated compared with normal endometrium (F=21.307，q=2.583-7.905，P＜0.01). Furthermore, the expression of COX＜0.05). No significant changes of COX（q=2.623,5.908P＜0.05）. There were no correlation between the mRNA expressions of 450arom and COX，and rAFS phases. There was a positive correlation between P450arom mRNA expression and COX（r=0.964，P＜0.01) and AM （r=0.813，P＜0.01）.ConclusionP450arom and COX mRNA expressions a