子宫内膜异位症组织SRC-1、ER与PR表达及意义_医学论文.docVIP

子宫内膜异位症组织SRC-1、ER与PR表达及意义_医学论文 子宫内膜异位症组织SRC-1、ER与PR表达及意义_医学论文 作者：欧阳江华 郭新华 高萍 夏玉芳 【摘要】 目的 探讨类固醇受体辅活化因子-1（SRC-1）、雌激素受体（ER） 、孕激素受体（PR）在卵巢子宫内膜异位症（EMs） 病人在位和异位内膜中的表达及其意义。方法 采用免疫组织化学SABC法,对45例EMs病人在位和异位内膜及42例对照组正常内膜中的SRC-1、ER、PR 进行染色，并利用图像分析系统测定其灰度值。结果SRC-1 在在位内膜中的表达显著高于异位内膜和对照组内膜，差异有显著性（t=8.210、7.260，0.05）；SRC-1在在位内膜增殖期和分泌期中的表达强度均显著高于相应期别的异位内膜及对照组内膜（F=58.641、7.650,q=4.162～12.052，0.01）。ER、PR在异位内膜增殖期的表达均显著低于同期对照组及在位内膜组（F=65.08、59.42，q=11.661～14.500，0.01）；对照组内膜中SRC-1、ER、PR在增殖期表达显著高于分泌期（t=5.909～10.183,0.01）。SRC-1、ER、PR 在EMs 的表达与rAFS 分期差异均无显著性（t=1.352～1.838,Pgt0.05）。在位内膜中SRC-1与ER的表达强度呈正相关（r=0.779,0.05）。结论 SRC-1、ER、PR 在在位和异位内膜中的表达异常可能与EMs的发生发展有关。 【关键词】 类固醇受体辅活化因子-1；受体,雌激素；受体,孕激素；子宫内膜异位症；免疫组织化学 　　［ABSTRACT］Objective To study the expressions and their significance of steroid receptor coactivator -1 （SRC-1）, ER and PR in eutopic and ectopic endometrium in patients with endometriosis （EMs）. MethodsUsing immunohistochemistry SABC method, the expressions of SRC-1, ER and PR in ectopic and eutopic endometrium of 45 EMs cases, and in 42 normal cases were examined and the gray scale detected by image-analysis system.Results The expression of SRC-1 in eutopic endometrium of EMs was significantly higher than that of ectopic endometrium and the normal control, the difference between them was significant （t=8.210,7.2600.05）. The expression of SRC-1 in proliferative and secretory phases of eutopic endometrium was significantly higher than that of the homologous-phase ectopic endometrium and the normal control （F=58.641,7.650q=4.162－12.0520.01）. The expressions of ER and PR in ectopic endometrium during proliferation were lower than that of concurrent control group and eutopic endometrium （F= 65.08,59.42q=11.661－14.5000.01）. The expressions of SRC-1, ER and PR in the control at proliferative phase were significantly higher than that in secretory phase （t=5.909－10.183,0.01）. The differences between the expressions of SRC-1, ER, PR in EMs, and rAFS staging were not significant （t=1.352－1.83