弥漫性大B细胞淋巴瘤bcl_医学论文.docVIP

弥漫性大B细胞淋巴瘤bcl_医学论文 弥漫性大B细胞淋巴瘤bcl_医学论文 作者：蒋会勇，陈愉，张三泉，朱梅刚，赵彤 【关键词】 弥漫 Correlation between bcl2/IgH gene rearrangement and GCB molecular subtype in diffuse large Bcell lymphoma 【Abstract】 AIM: To explore the correlation between bcl2/IgH gene rearrangement and germinal center Bcelllike (GCB) molecular subtype in diffuse large Bcell lymphoma (DLBCL). METHODS: Selfdesigned heminested PCR was used to detect bcl2/IgH gene rearrangement in 60 cases of DLBCL. Positive PCR products were cloned and sequenced. The tissue microarray of 60 specimens of DLBCL was prepared and the expressions of CD20, CD10, Bcl6 and MUM1 were investigated by immunohistochemical SP method. GCB and nongerminal center Bcelllike (nonGCB) were subclassified. RESULTS: Six of the 60 DLBCL were bcl2/IgH positive by conventional PCR method. Selfdesigned heminested PCR was used to amplify 6 positive cases again and bcl2/IgH gene was verified rearrangement in 5 cases by cloning and sequencing and 1 had the gene segment of BAC331191 of human chromosome 19. CD20 was positive in the 60 DLBCL cases. Positive expression rates of CD10, Bcl6 and MUM1 were 43.3%, 46.7% and 61.7% respectively. 32 (53.3%) were GCB and 28 (46.7%) were nonGCB. Bcl2/IgH gene rearrangementpositive cases were all GCB subtypes, which were significantly correlated with the expression of CD10 (P=0.012) and not with Bcl6 and MUM1. CONCLUSION: Selfdesigned heminested PCR can improve the detection accuracy of bcl2/IgH gene rearrangement. Bcl2/IgH gene rearrangement detection helps the DLBCL determination of GCB subtypes. 【Keywords】 lymphoma, Bcell, diffuse large；bcl2 gene rearrangement 【摘要】 目的: 探讨弥漫性大B细胞淋巴瘤（DLBCL）bcl2/IgH基因重排与分子亚型生发中心样（GCB）的相关性. 方法:采用自行设计的半巢式PCR对60例DLBCL的bcl2/IgH基因重排进行了检测，并对阳性产物进行克隆、测序. 同时采用免疫组化SP法在组织微阵列上同步观测CD20，CD10，Bcl6，黑色素瘤相关抗原（突变体）1 (MUM1)的表达,进行GCB和非生发中心样(nonGCB)分子分类. 结果: 经常规法扩增bcl2/IgH，有6/60例DLBCL bcl2/IgH阳性；在6例阳性样本中,采用本组设计的半巢式PCR扩增，经克隆及测序证实5例bcl2/IgH基因重排阳性，1例为人类第19号染色体BAC 331191基因的片段. 60例DLBCL CD20表达全部阳性；CD10，Bcl6， MUM