当归炭饮片质量标准研究_药学论文.docVIP

当归炭饮片质量标准研究_药学论文 当归炭饮片质量标准研究_药学论文 作者：杜天玺，郭延生，华永丽，杨洪申，秦亮，杨小虎，曲亚玲，魏彦明 【摘要】 　　目的建立当归炭饮片质量控制方法，为其质量标准的制定提供依据。方法观察当归炭饮片性状；对其粉末进行显微鉴别；根据《中国药典》规定的方法测定水分、灰分、酸不溶性灰分及其浸出物；采用薄层色谱法对其进行鉴别；基于当归炭饮片HPLC图谱，以阿魏酸峰为基准峰,计算阿魏酸的含量，对样品中指认的指纹峰以阿魏酸为参照，计算每个指纹峰以阿魏酸计的含量。结果当归炭和生当归相比，外观性状、显微结构发生了较明显的变化，组织颜色加深，淀粉粒被糊化，油细胞被破坏。水分、总灰分、酸不溶性灰分和浸出物测量平均值分别为3.3%，11.5%，4.1%和49.2%，确定了10个指纹峰，计算出指纹峰以阿魏酸计的含量。结论建立了当归炭饮片多指标成分的定量方法，方法具有可操作性，使药品质量控制更全面。 【关键词】 当归炭饮片； 质量控制； 定量方法； 阿魏酸 　　Abstract：ObjectiveTo establish the quality standard of carred Radix. Angelica Sinensis. MethodsTraditional identification, TLC and fingerprints of Carred Radix Angelica Sinensis were adopted to control quality of carred Radix Angelica Sinensis. The ten common peak were appointed as fingerprint peaks, all the fingerprint peaks were quantified grounded on the peak of Ferulic acid. Referring to China Pharmacopoeia (2005), water, ash, and extract were also detected. ResultsAppearance, microscopic structure of Radix Angelica Sinensis changed obviously, color of tissue deepened, starch granule gelatinized, oil cells were destroyed. The content of water, total ash, acid-insoluble ash and extract were 3.3%,11.5%,4.1%and 49.2%, respectively. Ten fingerprint peaks were defined, and quantified rested on the peak of ferulic acid. ConclusionA multicomponent quantitative method for the carred Radix Angelica Sinensis was established. The established method is feasible. The quality control standards of the carred Radix Angelica sinensis is normative, systematic and accurate. 　　Key words： Carred Radix Angelica Sinensis; Quality standards; Quantitative method; Ferulic acid 　　当归为伞形科当归属植物当归Angelica sinesis(Oliv.)Diels的干燥根［1］，主产于甘肃岷县，属甘肃地道药材，主要成分含挥发油和水溶性物质两大类。挥发油中以藁本内酯和正丁烯酉夫内酯为主要成分；水溶性物质以阿魏酸和丁二酸为主要成分［2］，是中（兽）医临床常用药，性温，味甘、辛，具有补血活血、调经止痛、润燥滑肠等功效。当归炭入药，最初见于明代方贤《奇效良方》成为“火化法”，之后清代又有“煅法”“烧法”的记录，如“烧末为灰”，但均需存性，当归炭至今仍为中医临床所选用［3］，由于当归本有活血作用，炒炭后又善止血，所以当归炭尤宜于瘀阻出血证。当归炭质量优劣直接关系到中医及中兽医临床的疗效，而目前还没有一个系统的质量控制的方法。因此，本文选取甘肃道地产区的当归，按照《甘肃省中药炮制规范》规定的方法炮制，采用传统鉴别和色谱指纹图谱结合的方法来控制当