模拟失重大鼠心肌细胞I Ca 对β┐受体激动剂的反应性_医学论文.docVIP

模拟失重大鼠心肌细胞I Ca 对β┐受体激动剂的反应性_医学论文 模拟失重大鼠心肌细胞I Ca 对β┐受体激动剂的反应性_医学论文 作者：程宏伟，张立藩，冯汉忠，程九华，余志斌 【关键词】 失重模拟 　　关键词: 失重模拟大鼠心肌/细胞学钙膜片钳受体，肾上腺素能β 　　摘 要:目的 研究模拟失重大鼠心肌细胞钙电流（ICa ）对β-肾上腺素受体激动剂的反应性变化. 方法 用尾部悬吊大鼠模型模拟失重（4wk），胶原酶分离心肌细胞，用膜片钳全细胞方式记录单个心肌细胞的膜电容和I Ca 等.给予β-肾上腺素受体激动剂（异丙肾上腺素），记录观察ICa 的反应性. 结果 对照组与悬吊组的ICa 密度无明显差别（Pgt0.05），ICa 电流密度-电压曲线基本一致加入异丙肾上腺素后，两组ICa 密度均明显增大，但是两组的增加幅度不同，悬吊组的增加幅度（84.3±8.2）%明显小于对照组（109.3±7.9）%（0.05）. 结论 模拟失重4wk大鼠心肌细胞的ICa 密度无明显改变模拟失重后心肌细胞ICa 对β-肾上腺素受体激动剂的反应性降低. 　　Keywords:weightlessness simulationratsmyocardium/cy-tologycalciumpatch-clamp techniquesrecep-tors，adrenergic，beta 　　Abstract:AIM To investigate the changes of responsiveness of calcium currents（I Ca ）toβ-adrenergic receptor agonist in cardiac myocytes of simulated ightless rats.METHODS The rat tail-suspension model was used to simulate weight-lessness（4wk）.Cardiac myocytes were isolated with colla-genase.Under whole cell recording mode of patch clamp technique，membrane capacitances and I Ca were recorded from the single ventricular myocytes.The responsiveness of I Ca was observed after stimulation by aβ-adrenergic receptor ag-nist（isoproterenol）.RESULTS I Ca densities did not show significant difference between control and suspended groups（Pgt0.05），and their ICa density-voltage curves were almost same.After isoproterenol was added，I Ca densities were obvi-ously increased in both control and suspended groups，but their increase percentages were not equal.The increase per-centage of I Ca density in suspended group（84.26±8.22）%was significantly less than that in control group（109.3±9.9）%（0.05）.CONCLUSION It is suggested that ICa densities of cardiac myocytes have no obvious changes in4wk simulated weightless rats.Responsiveness of I Ca toβ-adrener-gic receptor agonist significantly decreases after simulated weightlessness. 　　0 引言 　　关于失重/模拟失重下心血管系统β-肾上腺素受体反应性的变化，文献报道结果不甚一致［1-3］ .在评定心血管系统β-肾上腺素受体的反应性时，大多数文献均是在体条件下以心率等为指标，而我们知道β-肾上腺素系统在心肌兴奋与收缩的调制中起着非常重要的作用［4］ ，β-肾上腺素受体激活可引起心肌细胞内向钙电流增加，起到正性