欧亚旋覆花总黄酮对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究_药学论文.docVIP

欧亚旋覆花总黄酮对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究_药学论文 欧亚旋覆花总黄酮对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究_药学论文 【摘要】 目的观察欧亚旋覆花总黄酮（total flavones extracted from Inula britannica L， TFIB）对大鼠局灶性脑缺血-再灌注脑组织损伤的保护作用，并进一步探讨其作用机制。方法采用大脑中动脉栓塞法制作大鼠局灶性脑缺血再灌注损伤模型，缺血2 h，再灌注22 h后，以盐酸2, 3, 5三苯基四氮唑（TTC）染色法测定脑梗塞面积,比色法测定脑组织匀浆中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,采用“5分法”进行神经症状评分。 结果TFIB (25，50，100 mg· kg-1，ip) 能明显改善脑缺血-再灌注后大鼠神经症状,明显减小梗塞面积与全脑面积之比，提高缺血脑组织SOD活性，降低MDA含量。结论TFIB 对大鼠缺血-再灌注损伤大脑具有显著的保护作用,其作用机制可能与TFIB抗氧化作用有关。 【关键词】 欧亚旋覆花总黄酮； 脑缺血再灌注； 抗氧化 　　Abstract:ObjectiveTo investigate the protective effects of total flavones extracted from Inula britannica L.(TFIB) on cerebral ischemia -reperfusion injury in rats and its mechanism.MethodsThe model of focal reperfusion injury after cerebral ischemia in rats was made by middle cerebral artery occlusion. For 2h, followed by 22 h reperfusion. The infarction area of brain was assessed in brain slices stained with 2% solution of 2, 3, 5-triphenyl tetrazolium chloride. Spectrophotometric assay was used to determine the activity of superoxide dismutase ( SOD) and the content of malondialdehyde (MDA) in brain tissue. Neurological deficit was evaluated by using a 5-point neurological function scoring system.ResultsTFIB (25, 50, 100 mg·kg-1, ip) significantly decreased neurological deficit scores and reduced the percentage of infarction in the ip silateral hemisphere compared with the model group. TFIB significantly increased SOD activity and decreased MDA content.ConclusionTFIB provides significant protection against cerebral ischemia-reperfusion injury in rats. 　　Key words:Total flavones extracted from Inula britannica L.; Cerebral ischemia-reperfusion; Antioxidant action 　　缺血性脑损伤是以微循环下降为特征的神经系统疾病。临床上脑缺血发生后, 大部分患者还有血流再灌注现象发生, 当血流再灌注时会产生大量的自由基, 导致体内脂质过氧化反应, 形成一系列的脂质自由基及其降解产物。这些脂质自由基将进一步引起膜的流动性降低、通透性增高、线粒体肿胀、溶酶体损坏及释放。因此, 自由基损伤比单纯脑缺血损伤更加严重［1］。现有的许多钙拮抗剂只能治疗单纯脑缺血损伤，而对再灌注损伤无很好地治疗作用。研究证明自由基清除剂和抗氧化药物可以减轻缺血性脑损伤，产生神经保护作用［2，3］。黄酮类化合物具有清除自由基和抗应激反应的能力。欧亚旋覆花含有槲皮素、木犀草素、槲皮苷等多种黄酮类化合物，具有抗氧化活性［4，5］。然而有关欧亚旋覆花总黄酮( to