氨基水杨酸对三硝基苯磺酸诱导的大鼠结肠炎模型的影响_医学论文.docVIP

氨基水杨酸对三硝基苯磺酸诱导的大鼠结肠炎模型的影响_医学论文 氨基水杨酸对三硝基苯磺酸诱导的大鼠结肠炎模型的影响_医学论文 作者：张林，夏冰，程虹，李瑾，叶梅 【关键词】 结肠炎；三硝基苯磺酸；白细胞介素 【Abstract】 AIM: To investigate the effect of 4aminosalicylic acid (4ASA) on trinitrobenzene sulfonic acid (TNBS) induced colitis in rats and to clarify the related mechanism. METHODS: Colitis was induced in female SD rats by rectal administration of TNBS resolved in ethanol. The colitis rats were treated with 4ASANa with doses of 100 and 200 mg/kg respectively for 2 weeks. 5aminosalicylic acid (5ASA) and normal saline were used as control groups. The colonic inflammation including macroscopic and histological changes and myeloperoxidase (MPO) activity was evaluated. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in colonic mucosa were detected by biochemical methods. The expressions of interleukin1β (IL1β) and tumor necrosis factorα (TNFα) mRNAs in colonic mucosa were determined by a reverse transcription polymerase chain reaction (RTPCR) method. RESULTS: Compared with TNBS control group, the macroscopic and histological scores and MPO activity in 4ASA treated groups were decreased (0.05), SOD activity was increased (0.05), and the level of MDA in colonic mucosa was reduced significantly (0.05). The expressions of IL1β and TNFα mRNAs in colonic mucosa were also decreased significantly (0.05). The intracolonic administration with 4ASA had no more therapeutic effect than topical treatment with 5ASA. CONCLUSION: The topical treatment with 4ASA can significantly attenuate the colonic damage in TNBSinduced colitis in rats and it may be related to the antioxidant mechanism and the inhibition of the expression of proinflammatory cytokines including IL1β and TNFα. 【Keywords】 colitis trinitrobenzenesulfonic acid interleukin1β tumor necrosis factorα superoxide dismutase malondialdehyde Paminosalicylic acid 【摘要】 目的： 观察4氨基水杨酸（4ASA）治疗三硝基苯磺酸（TNBS）诱导结肠炎的疗效并探讨其作用机制. 方法： 直肠给予雌性SD大鼠TNBS诱导结肠炎，应用4ASANa（100, 200 mg/kg）对其进行治疗，并以5氨基水杨酸（5ASA）作为阳性对照，分别于1 wk及2 wk后取结肠