水杨酸钠给药对小鼠下丘核NGB mRNA和蛋白表达的影响_医学论文.docVIP

水杨酸钠给药对小鼠下丘核NGB mRNA和蛋白表达的影响_医学论文 水杨酸钠给药对小鼠下丘核NGB mRNA和蛋白表达的影响_医学论文 作者：尹时华，龚树生，鄢开胜，李穗，陈沛，陈广理 【关键词】 水杨酸钠 　　【Abstract】 AIM: To study the effects of sodium salicylate on the expression of NGB mRNA and protein in inferior colliculus neurons in mice. METHODS: Fortyeight Kunming mice were divided into four groups: control group (A) ［200 mg/(kg#12539d)］, ［300 mg/(kg#12539d)］, and ［450 mg/(kg#12539d)］ sodium salicylate administrated groups (B, C, D). The expression of NGB mRNA was measured by using RTPCR and the protein expression of NGB was measured by WesternBlot. RESULTS: The expression of NGB mRNA in control group increased remarkably than that in the other three sodium salicylate administrated groups (group B, C, D)（0.01）. The expression of NGB mRNA was the lowest in group D and the highest in group B among group B, C and D, with significant difference between group C and D (0.05). NGB protein with relative molecular mass 17 000 was observed in all groups by WesternBlot. The protein expression in group A was significantly higher than that in the other 3 sodium salicylate administrated groups. The protein expression of B group was the highest in group B and the lowest in group D in the three sodium salicylate administrated groups. CONCLUSION: Administration of sodium salicylate can decrease the expression of NGB mRNA and protein in inferior collicuLus neurons of mice and these changes are related to the dosage of sodium salicylate. 　　【Keywords】 sodium salicylate；mice；nucleus of inferior colliculus； neuroglobin 　　【摘要】 目的： 观察水杨酸钠给药对小鼠下丘核NGB mRNA和蛋白表达的影响. 方法： 成年健康昆明小鼠48只分为A，B，C，D四组，每组12只. A为对照组，B，C，D分别为水杨酸钠200, 300和450 mg/(kg#12539d)给药组. 给药15 d后处死检测；采用RTPCR检测实验小鼠下丘核区NGB mRNA的表达；WesternBlot分析NGB蛋白表达. 结果： ①对照组小鼠下丘核区NGB mRNA表达明显高于三个水杨酸钠给药组（0.01）；三个水杨酸钠给药组NGB mRNA表达比较以D组表达最低，B组表达最高，C组与D组比较有显著性差异（0.05）. ②WesternBlot检测结果显示实验各组均表达相对分子质量约为17 000的NGB蛋白. A组NGB蛋白表达明显高于其他各组；三个水杨酸钠给药组以B组表达最高D组表达最低. 结论：水杨酸钠可显著降低下丘核神经元NGB mRNA和蛋白表达；水杨酸钠给药对下丘核神经元NGB mRNA和蛋白表达与给药剂量有关. 　　【关键词】 水杨酸钠；小鼠；