淋巴管标记方法及标志物的探讨_医学论文.docVIP

淋巴管标记方法及标志物的探讨_医学论文 淋巴管标记方法及标志物的探讨_医学论文 【摘要】 目的 探讨不同标记方法及标志物在淋巴管检测中的价值，为研究肿瘤淋巴管的生成提供理想实验方法。方法 用5′核苷酸酶（5′）酶组织化学法对30例正常胃肠新鲜标本（胃及肠组织标本各15例）进行染色观察；用免疫组织化学PV9000法对30例相应胃肠标本石蜡包埋组织行血管内皮生长因子受体（VEGFR）和肾小球足突细胞膜黏蛋白（Podoplanin）染色观察。结果 淋巴管密度（LVD）:5′组LVD为6.78±1.16，Podoplanin组为6.85±0.84，VEGFR组为14.33±2.24。VEGFR组LVD明显高于5′组及Podoplanin组，差异均有显著性（t＝7.55、7.49，0.01），Podoplanin组与5′组比较差异无显著性（t＝0.07，Pgt0.05）。形态学观察：5′及Podoplanin标记的阳性淋巴管位于黏膜固有层及黏膜下层，管壁较薄，管腔内无红细胞；VEGFR染色阳性的淋巴管较多，位于黏膜固有层及黏膜下层，部分管壁较厚，管腔内见红细胞，且黏膜肌也呈阳性。染色背景：Podoplanin和VEGFR免疫组织化学染色背景清晰，能清楚显示阳性淋巴管与周围组织结构的关系；5′染色背景呈淡黄色，无法观察淋巴管周围的组织结构。结论 免疫组织化学技术下Podoplanin染色可以作为标记淋巴管的理想实验方法。Podoplanin是一种比较特异性的淋巴管标志物。 【关键词】 淋巴 5 核苷酸酶 血管内皮生长因子受体肾小球足突细胞膜黏蛋白 酶组织化学 免疫组织化学 ［ABSTRACT］ObjectiveTo compare different lymphatic vessel markers and labelling methods in order to find an ideal way for studying lymphangiogenesis in tumors. MethodsThirty normal gastrointestinal tissues were stained with 5′ were examined using immunohistochemistry technique. ResultsLymphatic vessel density (LVD) was different with different staining methods: The LVD of VEGFR3 group (14.33±2.24) was significantly higher than that of 5′Nase group (6.78±1.16) and podoplanin group (6.85±0.84) (t＝7.55，7.49；0.01). There was no significant difference between 5′（t＝0.07，Pgt0.05）. Lymphatics positively stained by 5′hinner and no red blood cells were found within lymphatics. VEGFR3 positively stained lymphatics were also found in lamina propria and submucosa. Part of the lymphatic walls were thicker, and red blood cells were found in the lymphtic duct. There was a clear background after podoplanin and VEGFR3 staining, which clearly showed the relationship between the lymphatics and the surrounding tissue, while 5′Nase staining showed wheat color background, which obscured the surrounding tissue structure of the lymphatics. Conclusion Immunohistochemistry staining with podoplanin was an ideal method for labeling tissue lymphatics. ［KEY WORDS］lymphatics 5′ 标记组织中淋巴管常用的方法有免疫组织化学染色检测血管内皮生长因子受体