激光照射对Jurkat细胞CD59活化途径的影响_医学论文.docVIP

激光照射对Jurkat细胞CD59活化途径的影响_医学论文 激光照射对Jurkat细胞CD59活化途径的影响_医学论文 【摘要】 目的 研究低功率He激光照射对Jurkat细胞CD59活化途径的影响。方法 将前期培养的Jurkat细胞随机平均分为3组:A组细胞不做任何处理, B组细胞每日定时进行7 min的He激光照射,C组细胞每日定时进行10 min的He激光照射。1周后用CD59单克隆抗体作用Jurkat细胞，用MTT法检测Jurkat细胞增殖情况，激光共聚焦方法观察细胞内Ca2+浓度变化，流式细胞术检测Jurkat细胞CD25表达。结果 B、C组Jurkat细胞增殖率、Ca2+浓度和CD25表达均高于A组(F=41.390～63.938，q=10.907～24.497，0.01) B组和C组间比较差异无显著性(Pgt0.05)。结论 低功率He激光照射可以促进Jurkat细胞经由CD59单克隆抗体刺激后的活化，增强活化信号的传导。 【关键词】 抗原，CD59Jurkat细胞激光 [ABSTRACT] Objective To study the effect of lown CD59 activate channel in Jurkat cells. Methods Prophase cultural Jurkat cells were evenly randomized to three groups: group A was served as normal control group B, Jurkat cells were irradiated 7 min daily group C, the cells were irradiated 10 min daily. One week later,CD59 monoclonal antibody was applied to act on Jurkat cells, which were detected by transformation test (MTT assay). Laser confocal technique was employed to observe the changes of calcium ion concentration (CIC) within the cells flow cytometry to detect the expression of CD25 in Jurkat cells. Results The growth rate of Jurkat cells, CIC and expression of CD25 in groups B and C were all higher than that in group A (F=41.390-63.938,q=10.907-24.497,0.01). As compared between groups B and C, the difference being not significant (Pgt0.05). Conclusion Lowpower HeNe laser radiation enhances the activation of Jurkat cells that stimulated by CD59, strengthening the conduction of activation signals. 　　[KEY WORDS] antigen, CD59 Jurkat cell laser 　　CD59是一种以糖基磷脂酰肌醇(GPI)锚着于细胞膜表面的糖蛋白,广泛分布于造血细胞、非造血细胞及多种组织细胞表面，除了已被广泛认知的补体调节功能外[1，还参与了T细胞的黏附及活化信号的传导。近年研究发现，肿瘤病人常常会出现免疫细胞CD59表达降低、活化能力减弱及免疫功能低下的情况[3，这就导致了肿瘤细胞逃避机体免疫系统的杀伤而持续生长，是造成肿瘤逃逸的重要因素之一。He激光具有光、热、磁、压等效应以及生物刺激作用，可以增强细胞的活化信号，促进细胞的增殖[5。本研究采用低功率He激光照射Jurkat细胞，以探讨低功率He激光对Jurkat细胞CD59的表达及活化信号转导的影响。现将结果报告如下。 　　1 材料和方法 　　1.1 材料与试剂 　　人白血病T淋巴细胞系Jurkat细胞由本室保存。 RPMI 1640 培养液及标准胎牛血清购于Gibco公司, MTT由中国医学科学院血液研究所提供,Hepes购于Solarbio公司,Fluo购于Biotium公司，FITC标记及辣根酶标记鼠抗人IgG购于北京中杉金桥公司。 HNZSQ型氦氖激光照射器为上海