烟酰胺干预UVB诱导豚鼠皮肤色素沉着的作用_药学论文.docVIP

烟酰胺干预UVB诱导豚鼠皮肤色素沉着的作用_药学论文 烟酰胺干预UVB诱导豚鼠皮肤色素沉着的作用_药学论文 作者：潘建英，张玉彬，帕它木·莫合买提，肖 萍，沈光祖，金锡鹏 【摘要】 目的 探讨烟酰胺对中波紫外线（UVB）致豚鼠皮肤色素沉着的抑制作用。方法 以亚红斑剂量UVB照射豚鼠背部10天建立皮肤无急性损伤色素沉着动物模型后，外涂基质和2.5%、5%、10%的烟酰胺4周，在此期间观察肤色改变和测定皮肤L值。涂药结束后取材，进行Masson－Fontana、冰冻切片Dopa染色和黑素细胞超微结构观察，观察黑素细胞数量、形态和功能的改变。结果 涂抹5%烟酰胺4周后，豚鼠皮肤变白，L值显著下降，多巴阳性黑素细胞数及黑素含量指数较对照组明显减少（0.05）。结论 5％烟酰胺对UVB诱导的色素沉着有明显的抑制，它主要是通过抑制黑素细胞的增殖和黑素细胞内黑素小体的合成而发挥作用。 【关键词】 烟酰胺；色素沉着； 黑素细胞 【Abstract】 Objective To investigate the inhibitory effect of nicotinamide on hyperpigmentation induced by UVB. Methods After hyperpigmentation was induced by UVB radiation on the back of brown guinea pigs， substance， 2.5%，5% and 10% nicotinamide were applied on the exposed skin for 4 weeks respectively. During this process， the changes of skin coloration were observed with naked eyes and L value were measured. Then the biopsy specimens were taken from the treated skin， sections were prepared for special staining and TEM examination and the number of melanocytes and melanin content index(MCI) were determined. Results After the application of 5% nicotinamide for 4 weeks， lightening effect was obviously observed with naked eyes and the L value decreased significantly. Compared with the substance control， the number of Dopa positive melanocytes and melanin content index were all significantly reduced (0.05) in the treated skin with 5% nicotinamide. Conclusion 5% nicotinamide is effective in lightening the UVB-induced pigmentation of guinea pig skin. This effect may be related to the declined proliferation of melanocytes and the inhibition of melanin synthesis in melanocytes. 【Key words】 nicotinamide；pigmentation；melanocyte 烟酰胺是近年来尝试用于美白和防晒化妆品中的一种化学物质［1，2］， 但对其效果和作用机制了解甚少。在国内，我们曾应用原代培养的人皮黑素细胞，探讨了烟酰胺抑制黑素合成的适宜浓度［3］，为进一步明确烟酰胺对皮肤色素沉着的抑制作用，本文以棕黄色豚鼠无急性损伤UVB致黑模型评价烟酰胺对皮肤色度的影响，并探讨了可能的作用机制。 1 材料与方法 1.1 实验动物 体重(355±12)g，健康成年雌性Hartley棕黄色豚鼠10只（上海银根动物养殖场）。 1.2 受试物 carpobol基质；将烟酰胺（分析纯，中国医药上海试剂供应站）溶于carpobol中分别配成2.5％、5％、10％三种浓度。 1.3 动物模型 在豚鼠背部选定4cm×4cm区域脱毛后用TL1