特发性血小板减少性紫癜患儿外周血T细胞亚型的分析_医学论文.docVIP

特发性血小板减少性紫癜患儿外周血T细胞亚型的分析_医学论文 特发性血小板减少性紫癜患儿外周血T细胞亚型的分析_医学论文 作者：肖红，刘仿，伍昌林，杨小猛【摘要】 　　T细胞亚型分化漂移可能与一些自身免疫病的发病机制有关。本研究旨在通过分析特发性血小板减少性紫癜（idiopathic thrombocytopenic purpura，ITP）患儿外周血Th/Tc、Th1/Th2、Tc1/Tc2细胞的平衡状态，探讨T细胞亚群失衡在ITP发病机制中的作用。收集30例ITP患儿外周抗凝静脉血，分离纯化得T细胞，以PE标记的抗CRTH2单克隆抗体和Cy5标记的抗CD4、CD8单克隆抗体作双色流式细胞术检测，分析ITP患儿Th/Tc、Th1/Th2、Tc1/Tc2比例的变化。结果显示：ITP患儿外周血T细胞与健康儿童相比，Th细胞百分率及Th/Tc比例明显下降（P﹤0.05），Tc细胞百分率无明显变化（P﹥0.05）；Th1及Th2细胞百分率明显下降（P﹤0.05），Tc1及Tc2细胞百分率无明显变化（P﹥0.05），Th1/Th2 及Tc1/Tc2比例明显升高（P﹤0.05）。结论： ITP患儿外周血可能存在细胞免疫功能低下及T细胞亚群漂移，Th1及Tc1细胞比例升高，呈明显Th1类细胞优势，此结果说明T细亚群分化异常在ITP发病过程中起着重要作用。 【关键词】 特发性血小板减少性紫癜；T细胞亚群； Th/Tc Th1/Th2 ；Tc1/Tc2；CRTH2 　　Analysis of Peripheral Blood T Cell Subsets in Children with Idiopathic Thrombocytopenic Purpura 　　Abstract The pathogenesis of some autoimmune diseases has been considered to be related to abnormal differentiation of T cell subsets. This study was aimed at investigating the change of Th1-like and Th2-like cells balance in ITP children，and analyzing the role of T cell subsets disequilibrium in the pathogenesis of ITP. Peripheral blood T cells were collected from 30 ITP patients，the T-cells were isolated and purified. The ratios of Th/Tc，Th1/Th2 and Tc1/Tc2 in peripheral blood T cells were analyzed by immunofluorescence staining and bicolor flow cytometry (FCM) in vitro. The results showed that as compared with the ratios of Th1/Th2 (48.76%±6.17%) and Tc1/Tc2 (18.90%±4.12%) in healthy children，the ratios of Th1/Th2 (56.21%±5.95%) and Tc1/Tc2 (23.09%±3.31%) in ITP children increased obviously. FCM analysis revealed that average percentages of Th，Th1，Th2，Tc，Tc1 and Tc2 were 22.31%±6.51%，21.92%±6.42%，0.39%±0.14%，31.12%±6.15%，30.95%±5.45% and 1.34%±0.84% in ITP children versus 39.24%±5.82%，39.01%±5.47%，0.80%±0.16%，30.25%±5.63%，28.72%±5.20% and 152%±0.68% in healthy children. The average percentages of Th，Th1 and Th2 decreased obviously，while the average percentages of Tc，Tc1 and Tc2 did not changed. It is concluded that the ratios of Th1/Th2 and