溴氰菊酯致大鼠慢性中毒的肝组织形态学观察_医学论文.docVIP

溴氰菊酯致大鼠慢性中毒的肝组织形态学观察_医学论文 溴氰菊酯致大鼠慢性中毒的肝组织形态学观察_医学论文 作者：马晓梅 吴梅 李杰 陈宗慧 石微 杜卫【摘要】 目的 探讨溴氰菊酯对大鼠肝组织结构的慢性损伤作用。方法 选取大鼠32只，随机分为空白对照组(Ⅰ组)、橄榄油对照组(Ⅱ组)、溴氰菊酯1.56 mg/kg组(Ⅲ组)、溴氰菊酯6.25 mg/kg组(Ⅳ组)，每组8只。Ⅲ、Ⅳ组连续6个月经口灌胃染毒, Ⅱ组只给予相当剂量的橄榄油，观察大鼠染毒期间行为学变化，测定肝脏器系数，肝组织切片经苏木精伊红染色和PAS染色，观察肝细胞形态学变化和肝细胞糖原含量的变化。结果 染毒组大鼠出现流涎、大汗、立毛等中毒症状。苏木精伊红染色显示Ⅲ、Ⅳ组肝细胞胞浆疏松，内可见脂滴，肝血窦淤血。染毒组大鼠肝细胞的糖原含量有不同程度降低，Ⅳ组与Ⅰ、Ⅱ、Ⅲ组相比，Ⅲ组和Ⅰ组相比差异有统计学意义(F=9.015，q=3.675～10.058，0.05)但Ⅲ组与Ⅱ组比较，差异无统计学意义(q=0.672，Pgt0.05)。Ⅲ、Ⅳ组肝脏器系数与其他各组比较，差异均有统计学意义(F=10.737，q=3.945～9.968，0.05)。结论 长期低剂量摄入溴氰菊酯可造成大鼠肝组织的慢性损伤，并具有剂量效应关系。 【关键词】 溴氰菊酯肝免疫组织化学糖原大鼠 [ABSTRACT] Objective To study the chronic injury effects of deltamethrin (DM) on rat hepatic tissue. Methods Thirty was given group 3, 1.56 mg/kg DM and group 4, 6.25 mg/kg DM. Rats in groups 3 and 4 were given DM through lavage for 6 months, and those in group 2, corresponding dose of olive oil, their behavior was observed during DM administration. Organ coefficient of liver was recorded. Histological changes of liver and the content of glycogen in hepatic cells were examined with HE staining and PAS staining. Results Toxic symptoms such as drivel, hidrosis, piloerection were observed in rats with DM administration. Lipid droplets within pale watery cytoplasm of hepatocytes and hepatic sinusoid congestion were found in rats of groups 3 and 4. Hepatocytic glycogen content was variously reduced, the differences were significant as compared between group 4 and groups 1, 2, and 3, respectively, or between groups 3 and 4 (F=9.015q=3.675-10.0580.05). But there was no significant difference in hepatocytic glycogen content between groups 3 and 2 (q=0.672，Pgt0.05). The organ coefficient of liver in groups 3 and 4 was significantly different from those in the other groups (F=10.737q=3.945-9.9680.05). Conclusion Longterm intake of lowdose deltamethrin may cause chronic injury of hepatic tissue in rats with a doseeffect relation. 　　[KEY WORDS] deltamethrin liver immunohistochemistry glycogen rats 　　溴氰菊酯商品名敌杀死, 是一种含有α氰