益气化瘀方及其拆方对大鼠椎间盘纤维环细胞凋亡相关因子的作用_医学论文.docVIP

益气化瘀方及其拆方对大鼠椎间盘纤维环细胞凋亡相关因子的作用_医学论文 益气化瘀方及其拆方对大鼠椎间盘纤维环细胞凋亡相关因子的作用_医学论文 作者：周泉, 王拥军, 施杞, 孙鹏, 周重建, 胡志俊, 刘梅 【关键词】 ,益气化瘀方 ［摘要］ 目的：研究益气化瘀方及其拆方药理血清对大鼠诱导凋亡椎间盘纤维环细胞bcl2、Bax及半胱氨酸蛋白酶8（caspase8）表达的影响。方法：用细胞免疫化学和积分光密度分析等方法比较分析益气化瘀方及其拆方益气方、化瘀方药理血清，以及胰岛素样生长因子1（insulinlike growth factor 1, IGF1）对抗Fas抗体（antiFas antibody）诱导凋亡大鼠椎间盘纤维环细胞bcl2、Bax及caspase8表达的影响。结果：益气化瘀方、益气方、化瘀方及IGF1血清处理组与凋亡模型组相比，bcl2表达升高，Bax及caspase8表达降低，均有统计学意义（0.01）；益气化瘀方组与益气方、化瘀方组比较，Bax表达明显降低，有统计学意义（0.05）。结论：益气化瘀方及其拆方可以调控大鼠椎间盘纤维环细胞内Bax、bcl2和caspase8表达，其延缓椎间盘退变的机制可能与调控细胞的凋亡相关因子有关。 　　［关键词］ 益气化瘀方 椎间盘 纤维环细胞 bcl2 Bax caspase8 　　Effects of Yiqi Huayu Recipe and its decomposed formulas on apoptosisrelated factors of anulus fibrosus cells in rats 　　ABSTRACT Objective: To study the effects of Yiqi Huayu Recipe and its decomposed formulasmedicated sera on expressions of bcl2, Bax and caspase8 of apoptotic anulus fibrosus cells in rats. Methods: Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi Huayu Recipe, Yiqi Recipe, Huayu Recipemedicated sera and insulinlike growth factor 1 (IGF1) on the expressions of bcl2, Bax and caspase8 of apoptotic anulus fibrosus cells in rats induced by antiFas antibody. Results: As compared with apoptosis group, bcl2 expression was higher, Bax and caspase8 expressions were lower in Yiqi Huayutreated, Yiqitreated, Huayutreated and IGF1 groups (0.01). As compared with Yiqitreated group and Huayutreated group, Bax expression was lower in Yiqi Huayutreated group (0.05). Conclusion: Yiqi Huayu Recipe and its decompsed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of bcl2, Bax and caspase8. 　　KEY WORDS Yiqi Huayu Recipe intervertebral disc anulus fibrosus bcl2 Bax caspase8 　　椎间盘退变主要表现为椎间盘细胞的凋亡和细胞外基质合成与分泌的减少,椎间盘细胞自分泌和旁分泌Fas配体触发凋亡。益气化瘀法是施杞教授根据大量临床和实验研究总结出来的治疗颈椎病的法则，本研究探讨其对椎间盘细胞凋亡相关因子的作用。 　　1 材料与方法 　　1.1 主要试剂、仪器与材料 达氏修正依氏培养基（Dulbecco’s modified Eagle’s Medium, DMEM），G