白细胞介素18、血管内皮生长因子-C及其受体3在大肠癌中的表达和在淋巴结转移中的意义_医学论文.docVIP

白细胞介素18、血管内皮生长因子-C及其受体3在大肠癌中的表达和在淋巴结转移中的意义_医学论文 白细胞介素18、血管内皮生长因子-C及其受体3在大肠癌中的表达和在淋巴结转移中的意义_医学论文 作者：陈昊 白玲 张昶 刘毅 孟健 【摘要】 目的 探讨白细胞介素18（IL-18）、血管内皮生长因子-C（VEGF-C）及其受体3（VEGFR-3）在大肠癌中的表达意义及与淋巴结转移的关系。方法 应用免疫组化MaxVision快捷二步法检测30例大肠癌组织中IL-18、VEGF-C、VEGFR-3表达情况，并以10例正常黏膜和10例淋巴结转移性大肠癌组织标本做对照。结果 IL-18、VEGF-C、VEGFR-3在大肠癌组织中的表达均明显高于正常黏膜（P＜0.05）；IL-18 及VEGF-C在淋巴结转移性大肠癌组织中的表达也高于正常黏膜，且IL-18与VEGF-C的表达呈正相关（P＜0.05）；VEGF-C在淋巴结有转移组的表达明显高于淋巴结无转移组（P＜0.05）。结论 大肠癌细胞可表达IL-18、VEGF-C、VEGFR-3，其表达与大肠癌的发生、发展有关；VEGF-C表达与大肠癌淋巴结转移显著相关，可作为判断患者淋巴结转移的重要指标。 【关键词】 大肠癌；血管内皮生长因子-C；血管内皮生长因子受体3；白细胞介素18；淋巴结转移性大肠癌 Abstract： Objective To investigate the expressions of IL-18, VEGF-C and VEGFR-3 in human colorectal cancer and their significance in lymphatic metastasis. Methods Immunohistochemical MaxVision method was used to detect the expression of IL-18, VEGF-C and VEGFR-3 in 30 specimens of colorectal cancinoma, 10 normal mucosa and 10 lymph node with metastatic cancer. Results The expressions of IL-18, VEGF-C and VEGFR-3 in colorectal carcinoma were significantly higher than that in normal mucosa (0.05). IL-18 and VEGF-C were higher in metastatic lymph nodes than in normal mucosa, with the two expressions positively correlated (P＜0.05). The expression of VEGF-C was higher in the lymph node with metastatic cancer than in that without (P＜0.05). Conclusion Colorectal carcinoma cells can express IL-18, VEGF-C and its receptor VEGFR-3, their higher expressions are correlated with the carcinogenesis and development of colorectal carcinoma. The higher expression of VEGF-C is closely correlated with lymphatic involvement, and thus could be used as an important predictive marker for lymphatic metastasis. Key words: colorectal neoplasm interleukin 18 vascular endothelial growth factor-C vascular endothelial growth factor receptor 3 lymph node metastasis 淋巴道转移是大肠癌进展的重要因素。淋巴管生成（lymphangiogenesis）作为淋巴道转移的分子机制，近年来越来越受到重视。新近研究表明，血管内皮生长因子-C（vascular endothelial growth factor-C, VEGF-C）可通过VEGF-C/VEGFR-3(vascular endothe