复方二黄颗粒对肾大部切除大鼠肾脏保护作用的实验研究-毕业论文.docVIP

分 类 号 学校代码  学号 密级 复方二黄颗粒对肾大部切除大鼠肾脏保护 作用的实验研究  A Dissertation Submitted in Partial Fulfillment of the Requirements for the Degree of Master of medicine Experimental studies on protective effect of the Erhuang compound remedy to the rats with subtotal excision of kidney Candidate : Huixuan Pan Major  : Nephrology Supervisor: Prof. Jianshe Liu Huazhong University of Science and Technology Wuhan, Hubei 430074, P. R. China May, 2008 目  录 摘  要 ···························································································1 ABSTRACT ···················································································3 前  言 ···························································································5 材料与方法 ··················································································7 结 果 ·······················································································10 讨 论 ·······················································································16 结  论······················································································20 参考文献 ···················································································21 综述 附录  慢性肾衰竭发生机制的研究进展 ··············································24 攻读硕士学位期间发表论文 ····················································38 致  谢 ·························································································39 摘  要 目的：观察复方二黄颗粒对 5/6 肾切除大鼠慢性肾衰竭的防治作用，并以肾脏组 织局部细胞因子为切入点探讨其作用机制。 方法：1. 成年雄性 SD 大鼠，随机分为假手术组和手术组。手术组采用两步法制 备 5/6 肾切除模型后，再随机分为模型对照组、尿毒清组、复方二黄制剂高剂量组、 复方二黄制剂低剂量组；分组后，各组大鼠分别给予不同的药物灌胃(假手术组、模型 对照组给与相应量的蒸馏水)共 8 周。在灌胃前、灌胃后第 4 周和第 8 周时分别将每只 大鼠称重，第 8 周留取 24h 尿液后取血、离心取上清、处死所有大鼠，分别测定尿量、 肾重，检测 24h 尿蛋白、血肌酐(SCr)、尿素氮(BUN)、甘油三酯(TG)、胆固醇(TC)； 留取肾组织进行 PAS 染色，于光镜下观察各组大鼠肾组织的病理变化；进行统计学分 析。 2. 取假手术组、模型对照组和复方二黄制剂组三组大鼠肾组织进行切片，采用 免 疫 组 化 法 检 测 肾 组 织 中 α- 平 滑 肌 肌 动 蛋 白 (α-SMA) 、 转 化 生 长 因 子 -β1 (TGF-β1)、纤维连接蛋白(FN)的蛋白表达，并进行统计学分析。 结果：1. 肾大部切除后，大鼠逐渐出现慢性肾衰竭，与模型对照组相比，复方二 黄颗粒可以改善