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122 Chinese Journal ofM icroecology, Feb2009, Vol21No2 : 1005376 ( 2009) 1 1, 2 1 于连林 ,靳利娥 , 鲍卫仁 ( 1. 太原理工大学 煤科学与技术教育部与山西省重点实验室, 山西 太原 030024; 2. 太原理工大学 化学工程 与技术学院, 山西 太原 030024) ( ), 3, , (), 1 1 , ; ; R446. 5 A Prelim inary screen of cellu loly tic ba cteria in rum en by m inim um m ed ium 1 1, 2 1 YU Lianlin, JIN Lie , BAO Weiren ( 1. K ey L a bora tory f or Coal S cience and T echnology of Shanx i P rov ince and M in istry of Ed ucat ion, Ta iyuan Un iv ersity of Tech no logy, Ta iyuan 030024, Ch ina 2. Institu te of Chem ical E ng ineering and T echnology, Ta iyuan Un iv ersity of T echnology, Ta iyuan 030024, Ch ina) A b stract O bjective To prelmi inarily screen celluloytic bacteria in rumen. M ethod The bacteria and fungi were isolated by wt o different culturemedia; The aerobic, facultative anaerobic and anaerobic microorganismswere isolated by three different culture methods; Then, the cellulolytic bacteriawere screened by the reselective culturemedium ( contai ningm icrocellulose). Resu lt A strain of anaerobic bacteria and a strain of anaerobic fungiwere screened and isolated. Conc lu sion Themethod usedwas smi ple and efficient for screening and isolating celluloytic bacteria in rumen. K ey w ords Rumen; Cellulolytic bacteria; Screen and isolate , ( ), 3 , , , ,, ,1850 () ,M ITCHERLICH , ; 1906, SEILLIERE , ; 1945, , ; [ 1] , 1 , 1. 1 材料 1. 1. 1 仪器 SP 150BZ , HS840u () , Y QLS30,, , 80% ,, ,