一株新属水平厌氧发酵性细菌的分离与鉴定.pdfVIP

, Journal of AnhuiA gri. Sc.i 2010, 38( 17): 8840- 8843 李占东 卢瑶 1, 2 1* 1 1 匡小珠 , 邱艳玲 , 师晓爽 , 朱玉伟 ( 1. , 266101; 2. , 100049) [ 目的]分离农业废弃物秸秆厌氧降解过程中重 糖类发酵性细菌, 并进行生理生化特性研究及系统分类鉴定[方法]利用亨 盖特厌氧滚管技术从江西水田稻草堆腐物中分离到一株厌氧细菌NM7,基于16S rRNA基因序列分析确定该菌株的系统进化地位[结 果]菌株 NM7为中温厌氧革兰氏阴性短杆菌, 能发酵多种单糖和二糖产丙酸乙酸和少量丁酸, 无氢气产生菌株基因组 DNA G+ C T 含量为 39mo%l , 16S rRNA 基因序列与Paludibacterprop ionicigenesW 4 的同源性最高,相似性为91. 6% [结论]综合生理生化特性和 系统发育分析, 菌株 NM7为拟杆菌门( acteroides)紫单胞菌科(Porphyromonadaceae)新型菌株,代表一个新属分支,命名为Saccharibrevi T bacter Jiangxiensis, 模式菌株为Saccharibrevibacter JiangxiensisNM7 16S rRNA 基因;厌氧发酵性细菌; 系统发育分析 Q 93 A 0517- 6611(2010) 17- 08840- 04 Isolation and Chara cterization of A N ov elA na erob ic F erm enta tion Bacterium KUANG X iaozhu et al (Q ingdao Institute of ioenergy and ioprocess Technology, Chinese Academy of Sciences, Q ingdao, Shandong 266101) A bstract [Objective] The ami was to isolate new anaerobic ferm entative bacteria involved in the anaerobic digestion of rice straw. [M ethods] Strain NM7was isolated from a rotten rice straw stack in Jian, JiangxiProvince, byHungate anaerobic rolltubem ethod. [Results] Strain NM7 was amesophilic, anaerobic Gramnegative bacterium. StrainNM7 cou ld fermentvarious sugarsand them ajor endproductsof glucosewere propi onate, acetate and a small amount of butyrate, whereasno hydrogenwas detected. The genom icDNA G+ C contentw as 39 mol%. 16S rRNA gene sequence analysis indicated that strain NM7 belonged to phylum of acteroides.Paludibacter prop ionicigenes w as themost smi ilar speciesw ith a low smi ilarity of 91. 6% . [Conclusion] On the basis of the physiological and phylogenetical data, strain NM7 represented a novel taxon in the fam ily of Porphyromonadaceae, forwhich the nam eSaccharibrevibacter Jiangxiensis gen. nov. , sp. nov,