人结肠癌细胞系肿瘤干细胞特性的初步研究.pdfVIP

# 1646 # , ×实验研究Ø 陈克力, 江恒, 陈建芳, 裴莉, 潘凤, 梁后杰 第三军医大学西南医院肿瘤科, 重庆400038 Characteristics of cancer stem cells in human coloncancer cell lines CH E N K e-li, JIA N G H eng , CH EN J ian-f ang , P EI L i, P A N Feng , L IA N G H ou-j ie Dep artment of Oncolog , Southwest H osp ital, Third M ilitar M ed ical Universit , Chongq ing 400038, P . R. China = : 本研究对8 种人结肠癌细胞 [ ABSTRACT] OBJECTIVE: T investigate the characteristics f 系的干细胞特性进 初步检测, 以寻找适合 c l n cancer stem cells in c l n cancer cell lines and l k f r suit- 分选结肠癌干细胞的细胞系: 利用流 able cell lines apply ing t is late c l n cancer stem cells. METH- 式细胞术, RT-PCR 检测 8 种结肠癌细胞中 ODS: The expressi ns f CD133 in eight cell lines were investiga- CD133 的表达情况; 利用条件培养观察8 种 ted by fl w cyt metry and RT-PCR. T he different cancer cells 细胞系的肿瘤细胞在无血清培养基中的生 w ere cultured in serum-free medium f r the study f their Bi l gi- 物学特性; 利用裸鼠成瘤模型检测成瘤能力 cal characterist ics. The tum r m del in nude mice was used t e- 和转移能力: CD133 在8 种细胞系中 valuate the tum rigenicity and metast asis f different cancer cells. 的表达有显著差异, 最高的H CT 116 细胞系 RESULTS: The expressi ns f CD133 in eight cell lines w ere sig- + 中CD133 细胞含量为( 91. 4 ? 5. 0) % ; 各种 nificantly different and the highest HCT 116 cell line c ntained 细胞系细胞均能在无血清培养基中增殖, 但 ( 9 1. 4 ? 51 0) % CD133+ cells. Eight types f cancer cells c uld 只有 HCT 116 细胞能形成典型的/ 神经球样 pr liferate in serum-free medium, but nly HCT 116 cells c uld 结构0 且具有较高的成瘤能力和转移能力 f rm the typical / neur sphere0 st ruct ure and had high tum rige- 同等细胞浓度下移