去铁酮大鼠血浆蛋白结合率的实验研究.pdfVIP

4 12 ( ) 2009 12 Vol.4 No. 12 JOURNAL OF SHANDONG UNIVERSITY( HEALTH SCIENCES) Dec. 2009 : 16 1- 554( 2009) 12- 0130- 03 巩泉泉, 刘萍, 徐华雷, 张雅楠, 贾晓静, 李玉彩 ( , 250012) : 目的 建立测定透析液中去铁酮( DFP) 浓度的高 液相色谱法( HPLC) , 观察DFP 的大鼠血浆蛋白结合率 方法 以体外方式, 采用平衡透析法模拟DFP 在大鼠体内与血浆蛋白结合的过程, 并用HPLC 法测定其透析液中 DFP 的浓度及血浆蛋白结合率结果 低中高 3 种浓度下, DFP 的大鼠血浆蛋白结合率范围为4. 25% ~ 5. 31% , 不同浓度组血浆蛋白结合率差异无统计学意义( P 0.05) 结论 DFP 血浆蛋白结合率较低, 属于低血浆 蛋白结合药物, 其主要以游离形式存在而发挥药 : 去铁酮; 平衡透析法; 血浆蛋白结合率; 高 液相色谱; 大鼠, Wistar : R115 :A Plasma protein binding rate of deferiprone in rats GONG Quan-quan, LIU Ping, XU Hua-lei, ZHANG Ya-nan, JIA Xiao-jing, LI Yu- cai ( Department of Chemistry and Microbacteria Detection, School of Public Health, Shandong University, Jinan 250012, China) Abstract: Obj ective To develop a HPLC method for determination of deferiprone( DFP) concentration in dialysate and to study the plasma protein binding rate of DFP. Methods With an external manner, we used an equilibrium dialysis method to imitate the binding process between DFP and plasma protein in vivo and determine the concentration of DFP and the plasma protein bind- ing rate of DFP. Results The plasma protein binding rate of DFP at low, middle and high concentrations was between 4. 25% to 5. 31% , and it was not significantly different in all groups ( P 0.05) . Concolusion The plasma protein binding rate of DFP is low. So DFP is a kind of low plasma protein binding rate drug, and most of the drug molecules act on a body with a free type. Key words: Deferiprone; Equilibrium dialysis; Plasma protein binding rate; High performance liquid chromatography; Rats, Wistar ( deferiprone, DFP) 99% ; , , [ 1] [ 2] [3] , ( 200812