受稻瘟病菌诱导的水稻OSBTB基因的分子特征和功能初探.pdfVIP

维普资讯 中国水稻科学 (ChineseJRiceSci)，2006，20(6)：577～582 577 http：／／www．ricesci．cn；http：／／www．ricescience．org 受稻瘟病菌诱导的水稻 OsBTB基因的分子特征和功能初探 姜玉新i 董海涛 庄杰云 郑康乐 李德葆 一 (浙江大学 农业与生物技术学 院 生物信息与基 因网络研 究组 ，浙江 杭州 310029； 中国水稻研 究所 国家水稻改 良中心 ，浙江 杭州 310006；通讯联系人 ，E—mail：lidh@hzcnc．corn,http：／／www．estarray．org) A Preliminary M olecular Characterization and FunctionalInvestigation on OsBTB Gene InducedbyM agnaporthegrisea inRice JIANGYu—xinI，DONGHal—tao 。ZHUANGJie—yun ，ZHENGKang—le ，LIDe~bao ‘ (BioinformaticsandGeneNetze,orkResearchGroup，CollegeofAgricultureandBiotechnology，ZhejiangUniversity，Hangzhou310029， China； NationalCenterforRiceImprovement，ChinaNationalRiceResearchInstitute，Hangzhou310006，China； C0rrP户0”d ”gauthor， E-mail：lidb@hzcnc．corn；http：／／www．estarray．org) Abstract：BTB／P()ZdomainislocalizedinN—terminalregionofthisprotein family，whichbelongstoCzH2zincfinger transcriptionalfactors． A protein encoded bv GMPfJZ gene contained thisdomain was localized in ceIlnucleus． A gene ((BTB)codingforaGM POZ-hkeproteindetectedinthenear—isogenlcrice(Oryzasatira I．subsp．indica)lines，H7R and H7S，infectedwithanisolateofMagnaporthegrisea ZB15，wassequenced andcharacterized．Northern andW esternblotting assayindicatedthat BTB wasinducedduringM ．griseainfectioninthericeisogeniclinesH7R andH7S，andexpresseddif— ferentiallyin theincompatibleandcompatibleinteractionbetweenriceandM ．grisea．QuantitativeRT—PCR resultsindicated representati0n 0f BTB gene expression was c0rresp0nding with the selected resistantand pathogenesis—related genes． Thoughttogether，itmightbeinvolvedin resistantreactioninducedbyM ．grisea． Keywords：BTB／POZdomain；Oryzasativa；Magnaporthegrisea；near—isogenlclines；OsBTBgene；molecularcharac— terization；genefunction 摘 要 ：报道