宜宾多粮浓香型酒厂内细菌多样性及分布的研究.pdfVIP

年第 期（总第 期）· 60 酿酒科技 2010 5 191 LIQUOR－MAKING SCIENCE ＆ TECHNOLOGY 2010 No．5(Tol．191) 宜宾多粮浓香型酒厂内细菌多样性 及分布的研究（二） 周瑞平，陈云宗，唐代云 （四川省宜宾市叙府酒业技术中心，四川 宜宾 644000 ） 摘 要： 对宜宾多粮浓香型酒厂内生产过程环境中分离到的24 株优势菌株进行DNA 提取扩增测序，对其 16S rDNA 序列分析表明，其中13 株为Bacillus 属，9 株分属于Lysinibacillus 属、Staphylococcus 属、Rummeliibacillus 属， Brevibacillus 属、Brachybacterium 属，还有2 株菌与数据库中模式菌株相似性低于97 ％，很可能代表着新类群。利用 现代生物技术探索对酿酒相关细菌优势菌株的分子生物学信息，对浓香型白酒酿造过程中优势细菌区系的变化进 行探索，探讨多粮浓香型白酒酿造过程中优势细菌对白酒风味物质的影响。 关键词： 微生物； 多粮浓香型白酒； 16S rDNA； 分析 ： ； ； ： ： （ ） 中图分类号 Q93-3 TS262.31 TS261.4 文献标识码 A 文章编号 1001－9286 2010 05－0060－05 Research on Microbial Diversity Distribution in Yibin Multiple-grains Luzhou-flavor Liquor Distillery ZHOU Rui-ping, CHEN Yun-zong and TANG Dai-yun (Technical Center of Yibin Xufu Liquor Industry Co.Ltd., Yibin, Sichuan 644000, China) Abstract: 24 dominant microbial strains were isolated from the production environment in Yibin multiple-grains Luzhou-flavor liquro distillery. DNA extraction and amplification and sequencing of those strains were carried out. The 16S rDNA sequence analysis indicated that 13 microbial strains were Bacillus, 9 microbial strains were Lysinibacillus, Staphylococcus, Rummeliibacillus, Brevibacillus and Brachybacterium respectively, and the similarity of the other 2 strains was lower than 97 % compared to type strains in databank and they were probably new type strains. We may make use of modern biotechs to explore molecular biology information of dominant strains related to liquor-ma