hNOK抗体的制备及肺癌组织中NOK的表达检测.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, March 25; 24(3): 480-484 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究简报 hNOK NOK 1 1 1 1 2 1 徐方 , 李颖华 , 王银银 , 任芳丽 , 刘力 , 常智杰 1 , , , 100084 2 , , 100005 : Novel Oncogene with Kinase-domain (NOK)是一个新的肿瘤相关基因, 其结构上 与受体蛋白相似, 具有一个蛋 白激酶结构域。过量表达NOK 会导致肿瘤的产生与转移。为了更进一步研究生理状态下NOK 的功能, 需要制备NOK 特异性的抗体。利用GST 融合蛋白制备了人源NOK 的多克隆抗体, 并进行了纯化。检测表明所得的抗体具有很高的滴 度, 能够特异性检测NOK 蛋白的表达。使用该抗体进行了人肺癌组织的免疫组化检测, 发现该抗体能够高效识别组织 内源性表达的NOK 蛋白, 可为肺癌的诊断提供依据。 : 蛋白激酶, 多克隆抗体, NOK, 肺癌 Preparation of Anti-hNOK Antibodies and Expression Examination of NOK in the Lung Cancer Tissues 1 1 1 1 2 1 Fang Xu , Yinghua Li , Yinyin Wang , Fangli Ren , Li Liu , and Zhijie Chang 1 State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China 2 Department of Microbiology and Etiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100005, China Abstract: Novel Oncogene with Kinase-domain (NOK) is a novel tumor-related gene, coding receptor like protein with a kinase domain. Overexpression of NOK leads to tumorigenesis and metastasis. To further study NOK function in physiological