RAPD分子标记在酱油生产菌系统发育分析中的应用.pdfVIP

508 2007 34(3) RAPD * * * 王 斌 潘 力 郭 勇 ( 510640) :3042(AS3951) AS3495 , 8 RAPD 6 : Primer1Primer5Primer6Primer7 Primer8Primer9, RAPD- PCR , 4~ 8 , 1~ 4 , RAPDPCR , RAPD :RAPD , , , :Q93964 :A 2007) Application of RAPD Molecular Marker in the Phylogenetic Analysis of Soy Sauce Strains* * * WANG in PAN Li GUO Yong (School fo Bioscience and Bioengineering , South China University of Technology , Guangzhou 510640) Abstract: RAPD analysis of eight soy sauce strains isolated from commercial soy sauce koji was done with random primers, using A sp ergillus oryz ae AS3951 and Asp ergillus soj ae AS3495 as controls. Six appropriate primers (Primer1, Primer5, Primer6, Primer7, Primer8, Primer9) for RAPDPCR werescreened from nine random primers, and repetitive experiments demonstrated that their RAPDPCR polymorphic patterns werestable.There were usually 4~ 8 bands in their RADPPCR patterns, where the number of the main bands was 1~ 4 and the secondary bands were abundant.The phylogenetic tree of these strains was reconstructed according to their RAPDPCRpatterns, and it basically corresponded to traditional morphological taxology, demonstrating that the application of RAPDmolecular marker in thephylogenetic analysis of soy sauce strains was feasible. Key words: RAPDmolecular marker, Soy saucestrain, Phylogenetic analysis, Polymorphism (Asp erg illus 1990 , f lavus Group) , PCR, ( ) ,