MTT法_CPE观察法用于药物细胞毒性实验的比较与分析_阎祖炜.pdfVIP

1552 　2007 10 　 14 　5 　Practical Preventive Medicine, Oct.2007, Vol 14, No.5 :1006-3110(2007)05-1552-03 MTT 法、CPE 观察法用于药物细胞毒性 实验的比较与分析 阎祖炜,朱　欣,李闻文 　　:　　MTT CPE 。 　　 MTT 、、Hela , SPSS TC , 。 　 50 　MTT CPE 。 　　MTT 。 :　M TT 法;CPE 观察法;药物毒性实验 :R965　　　　　:A Comparison andAnalysis of MTTAssay andCPE MethodinDrug Toxicity Trials　YANG Zu-wei, ZHUXin, LI Wen- wen (Department of Clinical Laboratory Science, Xiangya edical College, Central South University, Changsha 4 10013, Hunan) Abstract:　Objective　To compare and analyze the MTT assay and the CPE methed used indrug toxicity experiments. 　Methods　The MTT assay and the CPE method were separately used in the drug toxicity experiments to Hela cell of Mahuang, Huangqin and Ribavirin;the TC was calculated and evaluated by P software, and analyzed by statisitical 50 method.　Result　There was no statistically significant difference betweenthe MTT assay and the CPE method.　Conclu- sion　The MTT method is more simple and direct inthe drug toxicity experiments. ey words:　MTT assay;CPE method;Drugtoxicity trails 　　 100 ml, 1 g/ml, , 100 ℃30 min 。 MTT CPE 4 ℃。 。, [2] 1.1.2.2　　 , , , 100 g 10 15 h, 1.5 h 1 。、、 h, , , SPSS TC , 50 100 ml, 1 g/ml, , 100 ℃30 min 。 4 ℃。 1.1.2.3　　:100 mg/ml, 1　 , 1.1　材料 1.1.3　5 mg/ml (M TT )　 25 1.1.1　细胞　Hela mg 5 ml PBS , , , 0.22 μm 。 DMEM (Gibco), 10%,