不同诱导源诱导家蝇幼虫抗菌蛋白效果的比较.pdfVIP

4·18 · 中国媒介生物学及控制杂志2005 年 12 月第 16 卷第 6 期　Chin J Vector Bio Control ,December 2005 ,Vol. 16 ,No. 6 ·论著· 不同诱导源诱导家蝇幼虫抗菌蛋白效果的比较 刘晖 ,万启惠 ,贺莉芳 ,张曦 　　【摘要】　目的 　比较5 种诱导源所诱导产生家蝇幼虫抗菌蛋白的抗菌活性 ,观察不同诱导源对家蝇幼虫抗菌蛋白 产生的诱导效果。方法 　用金黄色葡萄球菌、大肠埃希菌、大肠埃希菌脂多糖、紫外线及热诱导家蝇幼虫 ,在诱导后不同 时间提取抗菌蛋白进行抑菌实验 ,观察不同诱导源诱导后抗菌蛋白产生的高峰时间 ,并比较各诱导组高峰时间产生抗菌 蛋白的抗菌活性。结果 　各诱导组诱导家蝇幼虫产生抗菌蛋白达最大活性时间为诱导后36～48 h (热诱导后家蝇幼虫 ) 很快化蛹 ,未观察抗菌蛋白产生高峰时间 ;金黄色葡萄球菌诱导后的抗菌蛋白抗菌活性最佳 ,热诱导后的抗菌蛋白抗菌 活性也较未诱导组好。结论 　各诱导源诱导后抗菌蛋白产生的高峰时间不同 ;细菌诱导的抗菌蛋白抑菌效果最好 ,热诱 导也是一种较理想的诱导方法 。 【关键词】　家蝇幼虫 ; 诱导 ; 抗菌蛋白 ; 抑菌活性 Comparison of the Effect of Different Inducements Inducing the Antibacterial Proteins in the Musca domestica Larvae 　L IU Hui , W A N Qihui , HE L if an g , et al . Zunyi Medical College , Zunyi 563003 , China 【Abstract 】　Objective 　To compare antibacterial activity of antibacterial protein in the M usca domestica larvae by five inducements and observe the effect of the different inducements. Methods 　The M . domestica larvae were treated by S . aureus , E . coli , L PS , ultraviolet radistion and heatshock. Antibacterial protein by five inducements were separated at different time ,detected their antibacterial activity to observe the peak time of antibacterial activity by five inducements ,and compared antibacterial activity at the peak of antibacterial protein by five inducements. Results 　 The peak of antibacterial activity appeared at 3648 h after induced by four inducements (Because many M . domestica larvae pupated ,we did not observe the peak of antibacterial activity by heatshock) . The antibacterial activity of antibacterial proteins by S . aureus was best ,and t