大肠埃希菌和肺炎克雷伯菌外排泵转录水平与耐药的关系.pdfVIP

2011 1 26 1 Laboratory M edici e, Ja uary 2011, Vol 26. No 1. 51 :( 2011) : R378. 2 : A 蒋燕群, 李 俐, 钱燕斐 ( , 200233) : , , 59, () ( ), ( PCR ) acrR , ( RT) PCR m arA , AmpC 46. 7% ( 7) acrAB , 51. 9% ( 14) acrA kp , , 3 acrR, 9 m arA 59 39( 66. 1% ) Am pC , acrR m arA acrAB : ; ; ; R elationship between the level of efflux pump in E sch er ich ia coli and K lebs iella p neum onia and drug resistance JIANG Yanqun, LI L ,i QIAN Yanf ei. (D epartmen t of C linical Laboratory, the S i th P eop les H osp ita,l Shanghai J iaotong Univers ity School of M edicine, Shanghai 200233, China) Abstract: Objective To i vestigate the relatio sh ip betw ee bacterial resista ce a d its active role of efflux pump system s, exp lore the i flue ce bewt ee the regulatory ge es of efflux pump i E scherichia coli a d the expressio level of its structure ge e, a d a alyze the resista t mecha ism. M ethods 59 strai s of E scherichia coli a d K lebsiella pneum onia were collected, a d the strai s w ere class ified i to two groups as expermi e tal group a d co trol group accordi g to the resista ce of ceftazidmi e. Them ultiplex polym erase chai reactio ( PCR) was used for the am plificatio a d segue ci g of the regulatory ge e acrR, a d the real tmi e ( RT) PCR w as used for the detectio of the expressio level of efflux pum p a d the regulatory ge e m arA. The threedmi e sio test w as used for the detectio of Am pC beta lactam ase. R esults 7 strai s of Escherich ia coli ( 46. 7% ) had h igh acrAB tra scriptio level i expermi e tal group, a d 14 strai s ofK lebsiella p neumonia ( 51. 9% ) had h igh acrAkp tra scr