黄铁矿、单质硫、亚铁离子对浸矿微生物产生胞外多聚物的影响.pdfVIP

Trans. Nonferrous Met. Soc. China 24(2014) 1171−1178 Effect of pyrite, elemental sulfur and ferrous ions on EPS production by metal sulfide bioleaching microbes Zhi-guo HE1,2, Yan-ping YANG1,2, Shan ZHOU1,2, Yue-hua HU1,2, Hui ZHONG3 1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; 2. Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha 410083, China; 3. School of Life Science, Central South University, Changsha 410083, China Received 17 April 2013; accepted 3 March 2014 Abstract: Extracellular polymeric substances (EPS) produced by acidophilic bioleaching microorganisms play an important role in the production of acid mine drainage and metal sulfide bioleaching. EPS mediate the contact between microbial cells and growth substrates, having a pivotal role in organic film formation and bacterium-substratum interactions. The production and chemical composition of EPS produced by seven bioleaching strains grown with different substrates were studied. Analysis of the EPS extracted from these strains indicated that the EPS consisted of carbohydrates, proteins and galacturonic acid. The contents of EPS, carbohydrates, proteins and galacturonic acid of EPS were largely related to the kind of strain used and culture condition. The results show that EPS productions of microbes grown with pyrite were significantly higher than those of microbes grown with sulfur or FeSO ·7H O. The highest EPS production of the seven acidiphilic strains was (159.43±3.93) mg/g, which was produced by 4 2