Analysis of SAGE Data-An Introduction.pptVIP

Analysis of SAGE Data:An Introduction Kevin R. Coombes Section of Bioinformatics Outline Description of SAGE method Preliminary bioinformatics issues Description of analysis methods introduced in early paper Review of literature: statistics and SAGE What is SAGE? Serial Analysis of Gene Expression Method to quantify gene expression levels in samples of cells Open system Can potentially reveal expression levels of all genes: “unbiased” and “comprehensive” Microarrays are closed, since they only tell you about the genes spotted on the array How does SAGE work? From ditags to counts Locate the