DNA Microarrays.pptVIP

Patrick Schmid DNA Microarrays Patrick Schmid CSE 497 Spring 2004 What is a DNA Microarray? Also known as DNA Chip Allows simultaneous measurement of the level of transcription for every gene in a genome (gene expression) Transcription? Process of copying of DNA into messenger RNA (mRNA) Environment dependant! Microarray detects mRNA, or rather the more stable cDNA What is a DNA Microarray? (cont.) How do we manufacture a microarray? Start with individual genes, e.g. the ~6,200 genes of the yeast genome Amplify all of them using polymerase chain reaction (PCR) “Spot” them on a medium, e.g. an