脂肪干细胞HLA分子表达与体外抑制淋巴细胞增殖的实验研究_崔磊.pdfVIP

1890 2005 7 20 85 27 N at lM ed J Ch in a, Ju ly 20, 2005, V ol 85, No27 HLA 崔磊 尹烁 杨平 刘波 张英 刘伟 曹谊林 ( ad ipose derived stem ce lls, AD SCs) , ( 3), 2, 5 HLA H LA 1 10 /ADSC , 2 3 4 5 ( PHA, 2 g /m l), ( 1 10 1 10 1 10 1 10 ) / , ; 2( L2, 05 ng / m l) , 1 102~ 5 / FN, H LA , HLA FN 48 h , HLA , H LA FN PHA , L2 , ; FN A DSC, , ; HLA ; , H um an ad ipose derived stem cells suppress lym phocyte prolifera tion induced by ce llular or non specif ic m itogen ic stim u li CUI Le,i YIN Shuo, YAN G P ing, LIU Bo, ZHANG Ying, LIU W e,i CA O YilinD ep artm ent of P la tic and R econ tructive Surgery, ShanghaiN inth P eop leH o p ita,l Shanghai Second M ed ical Univer ity, Shanghai Ti ue Eng ineering Re earch and D evelopm ent C enter, Shanghai 200011, China A b stract O bjective T o study mi m uno log ica l properties of ad ipose derived stem ce lls( ADSC ) and the ir in vitro mi m unom odulato ry effects on lym pho cy tes. M ethod s AD SC w as iso la ted from fat tissue by liposuc tion and culture expandedC ells at passage2 w ere observed for the expression of HLA , H LA by FAC s ana lysis Per iphera l b lood lym phocytes ( PBL) we re cultured w ith a llogeneic A DSCs a t dose of 1 5 2 3 4 5 10 A th irdparty AD SCs at do se of 1 10 1 10 1 10 1 10 w ere added to the ongoing wt oway m ixed lym phocy te reactions ( M LR ) for 6daysV alue of CPM ( count per m inute) w as ca lcu la ted w ith lum inenscence counterR esu lts F low cytom etry showed that und ifferen tiated ADSC s express HLA class but no t class A dd ition of interferon ( FN ) fo r 4