草鱼CRP基因全长cDNA的克隆及其表达分析.pdfVIP

35 6 () Vol.35 No.6 2009 12 Journal of Hunan Agricultural University (Natural Sciences) Dec 2009 文章编号：1007-1032(2009)06-0673-06 草鱼 CRP 基因全长 cDNA 的克隆及其表达分析 1 2 1 2 1* 3* 唐春华 ，杨 丹 ，左振华 ，黄小西 ，陈 韬 ，张东裔 (1. 410128 2. 410004 3. 410003) 摘 要：C-(CRP)EST (CK233056) cDNA (SMART-RACE) CRP 5′1 520 bp cDNA EST CRP cDNA889 bp1672 bp 74 bp 143 bp5′3′(open reading frame, ORF)CRP 224 N 15 RT-PCR CRP mRNA CRP 7 24 h CRP mRNA 5 CRP 48 h 关 键 词：C-; RACE RT-PCR 中图分类号：S965.112 文献标志码：A Molecular cloning and expression of the full length cDNA of C-reaction protein from grass carp 1 2 1 2 1* 3* TANG Chun-hua , YANG Dan , ZUO Zhen-hua , HUANG Xiao-xi , CHEN Tao , ZHANG Dong-yi (1.College of Veterinarian ，HNAU ，Changsha 410128 ，China ；2.Central South University of Forestry and Technology ， Changsha 410004 ，China ；3. Institute of Biotechnology ，Changsha College ，Changsha 410003 ，China) Abstract ：A 520 bp of 5’-end of CRP cDNA from grass carp ，was cloned using the primers based on EST sequence of CRP (CK233056) by SMART-RACE. This 520 bp 5’-end fragment was processed with the CRP EST and a full-length cDNA of CRP was achieved. CRP cDNA was found to contain an ORF of 672 nucleotides flanked by a 74 nucleotides of 5’-UTR and a 143 nucleotides of 3’-UTR. The deduced amino-acid sequence contained 224 amino acid residues with a 15 signal peptide of amino-acid residues in the N-terminal. To detect the mRNA expression of CRP in different tissues