苯并a芘诱导小鼠腹主动脉瘤形成机制研究.pdfVIP

江医学2014 年第36 卷第21 期 苯并[a]芘诱导小鼠腹主动脉瘤 形成机制研究 蒋凤春 季亢挺 隋向前 唐疾飞 【摘要 】 目的 探讨香烟烟雾重要成分苯并[a]芘（BaP ）诱导小鼠腹主动脉瘤（AAA ）形成的相关机制。方法 48 只7~9 月龄 C57BL/6N 小鼠分为对照组、血管紧张素Ⅱ（Ang Ⅱ）组、BaP 组、Ang Ⅱ/BaP 复合物组。5 周后取各组小鼠腹主动脉观察大体标本形态, 切片行HE 染色观察血管结构；免疫组化法检测各组靶血管基质金属蛋白酶-3（MMP-3 ）、核转录因子- κappaB （NF- κB ）的表达, POD 法TUNEL 检测血管平滑肌细胞凋亡情况。结果 大体标本显示Ang Ⅱ/BaP 复合物组腹主动脉瘤形成率为41.67% ，明显高于 Ang Ⅱ组腹主动脉瘤形成率25.00%（P 0.05 ），HE 染色提示动脉瘤形成处弹性纤维紊 血管壁显著变薄。免疫组化提示Ang Ⅱ/BaP 复合物组 MMP-3 及 NF- κB 的表达均较Ang Ⅱ组及BaP 组增加 [（0.48 ±0.13）% vs （0.24 ±0.16 ）% ，（0.34 ±0.10 ）% ；（0.42 ± 0.12 ）% vs （0.23 ±0.06 ）% ，（0.32 ±0.09 ）% ，均P 0.05]。Ang Ⅱ/BaP 复合物组平滑肌细胞凋亡较Ang Ⅱ组及BaP 组更明显[（30 ± 12）% vs（19 ±5 ）% ，（23 ±4 ）% ，均P 0.05]。结论 BaP 在Ang Ⅱ协同作用下可促进老龄C57BL/6N 小鼠形成AAA ，其机制可能与 BaP 通过NF- κB 促进MMP-3 的表达使弹性纤维紊 及平滑肌细胞凋亡有关。 【关键词】 苯并[a]芘 腹主动脉瘤 基质金属蛋白酶-3 核转录因子- κappaB 平滑肌细胞 Benzo ［a］pyrene (BaP) promotes abdominal aortic aneurysm formation in mice and its mechanism China 【Abstract 】 Objective To investigate the effect of benzo a ］pyrene (BaP) on the formation of abdominal aortic aneurysm (AAA) in mice and its mechanism. Methods C57BL/6N mice aged 7 ~ 9 months were divided into four groups, control group, angiotensin Ⅱ(Ang Ⅱ) group, BaP group and Ang Ⅱ/BaP group. After 5 weeks of treatment of various agents the abdominal aor- tas of mice were taken. Aortic tissues were subject to HE, immunochemistry staining for evaluation of vascular wall structure, the expression of matrix metalloproteinase-3, nuclear factor- κB and the apoptosis of vascular smooth muscle cells. Results Ang Ⅱinfusion with BaP injection induced AAA in 41.67% of mice (versus 25% in Ang Ⅱgroup, P 0.05). Histological analysis of the aortic wall showed severe damage to the media