尖顶羊肚菌对急性酒精性胃黏膜损伤保护作用研究.pdfVIP

Mycosystema    菌 物 学 报 15 March 2011, 30(2): 319-324 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 尖顶羊肚菌对急性酒精性胃黏膜损伤保护作用研究 2 2 2 2 2 1* 罗霞 魏巍 余梦瑶 江南 许晓燕 郑林用 1 四川省农业科学院 成都 610066 2 四川省中医药科学院 成都 610041 摘 要：研究尖顶羊肚菌菌丝体水提液对酒精引起的大鼠急性胃黏膜损伤的保护作用。以 95%乙醇诱导的大鼠胃黏膜损伤为模 型，测定各组胃黏膜损伤指数，并测定胃黏膜超氧化物歧化酶（SOD）、丙二醛（MDA ）、谷胱甘肽（GSH ）含量，采用幽门 结扎法，测定大鼠胃酸、胃蛋白酶与胃黏液分泌的量。结果表明羊肚菌中、高剂量能明显降低胃黏膜损伤指数（p ＜0.05 ）；羊 肚菌不能抑制胃酸的分泌（p ＞0.05 ），但是能增加胃蛋白酶与胃黏液的分泌（p ＜0.05 ）；95%乙醇能引起胃黏膜 SOD 活性与 GSH 的降低，MDA 含量的增加，给予羊肚菌能明显抑制这一现象。结果说明羊肚菌对急性酒精性胃黏膜损伤的保护作用是与 增加胃黏液分泌与提高机体抗氧化能力有关。 关键词：尖顶羊肚菌，胃黏膜损伤，超氧化物歧化酶（SOD），丙二醛（MDA ），谷胱甘肽（GSH ），胃蛋白酶 The gastric protective effects of Morchella conica on the ethanol- induced gastric mucosal lesion in rats 2 2 2 2 2 1* LUO Xia WEI Wei YU Meng-Yao JIANG Nan XU Xiao-Yan ZHENG Lin-Yong 1Sichuan Academy of Agricultural Sciences, Chengdu 610066, China 2Sichuan Academy of Chinese Medicine Science, Chengdu 610041, China Abstract: The effects of Morchella conica mycelial extract on the ethanol-induced gastric mucosal lesion of rats in vivo were investigated. The gastric mucosal lesion was induced by 95% ethanol, the lesion index was measured and the superoxide dismutase (SOD), malonaldehyde (MDA), glutathione (GSH) content in the gastric issues were tested. The gastric acid, pepsin and gastric mucus were detected in ligated pyloru rats. The result showed that the middle dose and high dose of M. conica mycelial extract could reduce the lesion index of gastric mucosal (p 0.05); M. conica did not inhibit the gastric secretion (p 0.05) but promoted the pepsin and gas