mRNA差别显示技术及其在植物基因研究中的应用.pdfVIP

第 25 卷 　第 2 期 北 　京 　林 　业 　大 　学 　学 　报 Vol. 25 , No. 2 2003 年 3 月 JOURNAL OF BEIJ ING FORESTRY UNIVERSITY Mar. , 2003 mRNA 差别显示技术及其在植物基因研究中的应用 马月萍 　戴思兰 (北京林业大学园林学院) 摘要 　mRNA 差别显示技术是近年发展起来的一种用于分离和克隆正常与异常细胞之间差异表达基因的 PCR 方法 , 是目前筛选差异表达基因的有效方法之一. 自问世以来在动物遗传育种方面取得了很大的成绩,广泛用于人类及其 他动物生长发育基因调控、基因克隆的研究. 在植物基因研究方面应用起步较晚 ,现已开始用于植物基因分析、杂种 优势机理等方面的研究. 在此 ,该文介绍了 mRNA 差别显示技术的原理、步骤、优缺点及其发展 ,并对其在植物特异基 因分离方面的研究及其应用前景做了探讨. 关键词 　mRNA 差别显示 , 植物 , 特异基因 中图分类号 　Q9432 Ma Yueping ; Dai Silan. Technique of mRNA differential display reverse transcription PCR and its appli cation in gene research of plants. Journal of Beijing Forestry University (2003) 25 (2) 81～84[ Ch , 27 ref. ] School of Landscape Architecture , Beijing For. Univ. , 100083 , P. R. China. The mRNA differential display reverse transcription PCR (DDRTPCR) is a technique of cloning genes which are expressed differently between the good cells and bad cells and is an effective method too. It has been widely used in genetic regulating and breeding of animals and gained important achievements. DDRTPCR was used later in the research of plant genes , and it has been used to analyse gene and heterosis principle of plants now. The principles of DDRTPCR technique was introduced in this paper and its procedure , strong and weak points were described too. Furthermore ,this paper discussed the application of the technique on the research of plants. Key words 　differential display reverse transcription PCR (DDRTPCR) , plant , special gene 　　真核生物一般有 10 万个不同的基因 ,而在单个 景. 细胞中 ,仅有约 15 %的基因得到表达 ,产生约15 000 1 　mRNA 差别显