一株产紫杉醇菌的分离及其代谢产物对HeLa细胞抑制作用的研究.pdfVIP

Mycosystema 15 September 2008, 27(5): 735-744 jwxt@ ISSN1672-6472 CN11-5180Q ©2008 Institute of Microbiology, CAS, all rights reserved. Taxus cuspidata 181 TLC HPLCMS 1 HD181-23 206.34µg/LHD181-23 HD181-23 Botrytis sp.HeLa HeLa Isolation of a taxol-producing endophytic fungus and inhibiting effect of the fungus metabolites on HeLa cell ZHAO Kai ZHAO Li-Fei JIN Yuan-Yuan WEI Hai-Xia PING Wen-Xiang * ZHOU Dong-Po Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, China Abstract: One hundred and eighty one purified endophytic fungal strains were isolated and screened from inner bark (phloem-cambium) and xylem of Taxus cuspidata. These strains were fermented and then the fermented solution was extracted using methanol and ethyl acetate. The extracts were analyzed by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and mass spectrum (MS), using No. o. GA02C101 No. 2005AFQXJ063No. 1152HZ06 * Corresponding author. E-mail: zhoudp2003@ 2007-10-242008-02-19 736 Mycosystema standard taxol as control. The results showed that strain HD181-23 can produce taxol with output of 206.34µg/L. Morphological observation indicates that HD181-23 belongs to the genus Botrytis, and the fungus is tentatively identified as Botrytis sp. The purified products of the fermented extracts have significant inhibitive effects on HeLa cell. Key words: endophytic fungus, Botrytis sp., Taxus cuspidata, fermented extract taxolWani 1971Taxus brevifolia Nutt.