荧光定量PCR检测大肠癌患者CEAmRNA表达研究.pdfVIP

·42· 荧光定量PCR检测大肠癌患者CEAmRNA表达的研究+ 徐兰1，李晓霞2，郭玉杰1。亢澜涛1 1辽宁省肿瘤医院，辽宁沈阳 110042；2中国医科大学附属第四医院，辽宁沈阳110032 【摘要】 目的：通过检测大肠癌病人外周血及淋巴结中CEAmRNA的相对表达量，建立外周血中定量检测 CEAmRNA的方法。方法：采用逆转录一荧光定量PCR技术检测lO例正常人和40例大肠癌病人外周血及淋 正常对照组lO例无1例阳性，两者差异有统计学意义(P0．01)。34例大肠癌患者手术前外周血CEAmR— NA表达阳性率较高，且显著高于淋巴结中表达阳性率，可用于大肠癌患者的术前诊断。 【关键词】CEAmRNA，逆转录一实时定量PCR，外周血，淋巴结 QuantitativeDetection0fthe ofCEAmRNAinPaNentsWjtflInte洲naI expression Cancer RT—PCR theMethodofReaI—time by establishamethodfor detectionofCEAmRNA inthe 【Abs纽aa]Objective：To quantitative ExpressionPeripheral BloodAnd nodesofPatientswithIntestinal RT—PCRWagtakentodetectthe lymph Cancer．Methods：Real—time as Bloodof individualsand40 CEAmRNA in node．aswellinthe 10normal comparativeexpressionlymph peripheral is 87．5％in BIoodin40 CEAmRNA patients．Results：CEAmRNAExpressed penipheral preoperativepatients．IIO Wagdetectedin10normalindi“duals．in34 is 88．2％in preoperativepatients．c】队mRNAExpressed peripheral of PCRisestab． Blood，anditis 32．3％in node．Conclusion：ThemethodSYBRGREENreal—time expressed lymph 1ishedforthedetectionofCEAmRNAinPatientswithIntestinalCancer．TherateofCEAmRNA expression positivitv is in bloodof thanthatin Nodes．Itisusefulforthe relativelyHisherperipheralpreperativepatients lymph preperative of withIntestinal diagnosis Cancer． patients