聚谷氨酸-明胶交联生物胶的制备的研究.pdfVIP

M035 聚谷氨酸－明胶交联生物胶的制备研究 王欣，陈国广， 韦萍 （南京工业大学制药与生命科学学院，南京 210009 ） 摘 要 本文研究了用水溶性碳化二亚胺（WSC ）交联的聚谷氨酸－明胶生物胶的制备和组织粘 附性。明胶与不同分子量的聚谷氨酸交联制备的生物胶，在25℃明胶浓度低于自发胶凝浓度的情 况下，胶凝时间随聚谷氨酸和明胶的浓度及WSC 的浓度增加而增大，低分子量聚谷氨酸与明胶 － 、 － 的胶凝时间短于高分子量聚谷氨酸，在聚谷氨酸浓度20 mg ml 1 、明胶10 mg ml 1、交联剂WSC 120 mg ml－1 时，其胶凝时间小于30s，和纤维蛋白胶相当。WSC 交联的聚谷氨酸－明胶生物胶 的粘连强度优于蛋白纤维胶。所以聚谷氨酸－明胶生物胶是一种安全有效的生物胶。 关键词 聚谷氨酸 明胶 生物胶 水溶性碳化二亚胺 Studies on preparation of the PGA-gelatin biological glue WANG Xin，CHEN Guo-guang， WEI Ping （ Nanjin g University of Technology, Nanjing 210009, China ） Abstract The preparation and the tissue adhesion property of the polyglutamic acid(PGA)-gelatin biological glue was studied in the paper. The study focused on the WSC-formed biological glue from the gelatin whose aqueous solution did not spontaneously set to a gel at 25℃ and the different-molecular-weight PGA. At polymer concentration lower the incipient gelation concentration, the gelation time by the WSC-cross-linked PGA-gelatin biological glue increased with an increase in the concentration of PGA and gelatin and WSC, and the gelation time by the WSC-cross-linked low-molecular-weight PGA-gelatin biological glue is shorter than the gelation time by the WSC-cross-linked high-molecular-weight PGA-gelatin － 、 1 biological glue .when the concentration of PGA is 20 mg ml , the concentration of gelatin is 10 － 1 － 1 mg ml and the concentration of WSC is 120 mg ml , the gelation time by the WSC-cross-linked PGA-gelatin biologic