实验一、随机区组设计和拉丁方设计分析.docVIP

实验一、随机区组设计和拉丁方设计分析 一、随机区组设计分析 1. 用二氧化硅50mg对大鼠染尘后，测量不同时期全肺湿重的变化。试验采用完全随机平衡设计，单因素3个水平（数据文件为ExpDesLab_1.xls）。 1）用Anova方法比较3个时期(1月、3月、6月)的全肺湿重有无显著差别，列出方差分析表。 2）对3个时期的全肺湿重进行多重比较（LSD、Tukey和Bonferroni）并对比较结果进行分析。 2. 研究4个不同油菜品种在发芽和苗期对田间水分含量的敏感性，试验共测定7个指标，分别为发芽势、发芽率、发芽指数、平均发芽天数、根系活力、叶绿素含量和过氧化物酶（POD）活性。试验采用完全随机区组设计，2个水分水平，3个区组（数据文件为ExpDesLab_1.xls）。 1）用Anova方法对7个指标的数据进行分析，列出方差分析表，从中找出哪些指标对于4个品种来说是存在显著差异的。 2）对各个指标的4个品种表现进行多重比较（LSD、Tukey、Scheffe和Bonferroni方法）。 3）根据上面的分析结果，找出一个比较抗水渍的油菜品种（发芽势、发芽率、发芽指数、根系活力、叶绿素含量和过氧化物酶活性越高，平均发芽天数越短，品种越抗水渍）。 二、拉丁方设计分析 为研究5个不同剂量的甲状腺提取液对豚鼠甲状腺重的影响，考虑到鼠的种系和体重对观测指标可能有一定的影响，设计试验时，最好将这２个重要的非处理因素一并安排。所以试验采用拉丁方设计，数据如下： 种系 甲状腺提取液的剂量(字母)与甲状腺重(g/200g体重) I II III IV V 1 C 65 E 85 A 57 B 49 D 79 2 E 82 B 63 D 77 C 70 A 46 3 A 73 D 68 C 51 E 76 B 52 4 D 92 C 67 B 63 A 41 E 68 5 B 81 A 56 E 99 D 75 C 66 Experiment I Analysis of Randomized Block Design and Latin Square Designs 1. Analysis of Randomized Block Design (1) Mice were treated with 50mg SiO2 , and then lung’s wet weight in different stages were measured. Complete randomized design was used in the experiment, including single factor with 3 levels (data in ExpDesLab_1.xls). (a) Using ANOVA method to analyze significant difference of lung’s wet weight in 3 stages (1, 3, 6 month) and Listing ANOVA table. (b) Performing the multiple comparisons on the performance of 3 stages using LSD, Tukey and Bonferroni. Listing the compared results and explaining them. (2) Four varieties of rape were employed to study the sensitivity of different varieties on field water content during germination and seedling establishment. 7 parameters were obtained during experiment, including germination energy, germination percentage, germination index, mean germination day, root energy and POD. Randomize block design was used in experiment, including 2 level of water, 3 blocks (data in ExpDesLab_1.xls). (a) Using ANOVA method. List ANOVA table, analyze significance of each effects, and find out which indices have signific