肾气丸“阴中求阳”配伍对方中乌头碱和次乌头碱含量的影响_药学论文.docVIP

肾气丸“阴中求阳”配伍对方中乌头碱和次乌头碱含量的影响_药学论文 肾气丸“阴中求阳”配伍对方中乌头碱和次乌头碱含量的影响_药学论文 作者：范明威，陈炜璇，卓廉佳，李政木，吴勇 【摘要】 　　目的测定肾气丸各拆方组水煎液中乌头碱、次乌头碱的含量，分析肾气丸“阴中求阳”配伍对方中乌头碱、次乌头碱含量的影响。方法将肾气丸组方药物拆方分为补阳组-1、补阳组-2、补阴组-1、补阴组-2、阴阳双补组-1和阴阳双补组-2（即肾气丸全方），采用高效液相色谱法测定肾气丸各拆方组水煎液中乌头碱、次乌头碱的含量。结果各组水煎液中均未检测出乌头碱含量，阴阳双补组-2、阴阳双补组-1中次乌头碱含量均低于补阳组-1、补阳组-2，阴阳双补组-2中次乌头碱含量低于阴阳双补组-1，且都具有统计学意义。 结论肾气丸“阴中求阳”配伍能减小水煎液中次乌头碱的含量从而有降低毒性的作用。 【关键词】 肾气丸 阴中求阳 乌头碱 次乌头碱 高效液相色谱法 　　Abstract：ObjectiveTo detect the content of aconitine and hypaconitine in decoction of each dismantled Shenqi-Pill drug group, and analyze the influence of compatibility of Shenqi-Pill reinforcing Yang from Yin on the content of aconitine and hypaconitine in each decoction.MethodsDivided the drugs of Shenqi-Pill into Invigorating Yang Group Ⅰ, Invigorating Yang Group Ⅱ, Invigorating Yin Group Ⅰ, Invigorating Yin Group Ⅱ, Invigorating Yin-Yang Group Ⅰ, Invigorating Yin-Yang Group Ⅱ（the whole Shenqi-Pill）. And to measure the content of aconitine and hypaconitine in each dismantled Shenqi- Pill drug decoction by using the method of high performance liquid chromatogram.ResultsThe content of aconitine can not be detected in each group. The contents of hypaconitine in Invigorating Yin-Yang Group Ⅱ and Invigorating Yin-Yang Group Ⅰ are both lower than those in Invigorating Yang Group Ⅰ and Invigorating Yang Group Ⅱ. Meanwhile, the content of hypaconitine in Invigorating Yin-Yang Group Ⅱ is also lower than that in Invigorating Yin-Yang Group Ⅰ. All of these possess statistical significance. ConclusionThe content of hypaconitine in the decoction can be lowered through compatibility of Shen-qi Pill “reinforcing Yang from Yin” and the toxicity is minished. 　　Key words：Shenqi-Pill; Reinforcing Yang from Yin; Aconitine; Hypaconitine; High performance liquid chromatogram 　　肾气丸出自东汉张仲景的《金匮要略》，由附子、桂枝、干地黄、山茱萸、山药、丹皮、泽泻、茯苓等8味药物组成，具有补肾助阳的功效，是治疗肾阳虚证的有效经方。本方在大量滋阴补肾药物之中加入少量的附子、桂枝以达到微微生火、少火生气的目的，体现了“阴中求阳”的配伍思想，是方剂配伍理论中值得探讨研究的课题。本研究通过将肾气丸组方药物进行拆分，测定各个拆方组水煎液中乌头碱、次乌头碱的含量，并比较其含量变化。现报道如下。