脾虚证与胃窦D细胞的关系_医学论文.docVIP

脾虚证与胃窦D细胞的关系_医学论文 脾虚证与胃窦D细胞的关系_医学论文 作者：张航向　任平　黄裕新　李源　黄熙　刘芳 【关键词】 脾虚 关键词: 脾虚生长抑素四君子汤肠内分泌细胞免疫组织化学医学，中国传统 摘 要:目的 探讨脾虚证与生长抑素（somatostain，SS）内分泌细胞（D细胞）数量变化的关系. 方法 用免疫组化链酶亲合素过氧化酶复合技术（SABC）对利血平脾虚大鼠模型胃粘膜和患者胃粘膜组织的D细胞染色观察各组D细胞的形态和数量变化. 结果 和对照组相比，脾虚大鼠模型7d组D细胞数升高（43.4±3.1vs78.6±4.6，0.01），强阳性细胞率下降［（34.6±2.5）%vs（24.1±3.5）%，0.01］脾虚大鼠模型14d组D细胞强阳性细胞率升高［（34.6±2.5）%vs（46.4±9.0）%，0.05］四君子汤治疗组大鼠与正常对照组间D细胞数（43.4±3.1vs41.7±3.7）及强阳性细胞率无显著差异［（34.6±2.5）%vs（33.1±1.7）%，Pgt0.05］.患者脾虚组D细胞阳性数（70.4±10.1），（38.1±6.4），（45.4±8.6），（0.01）及强阳性率较胃热、肝火上炎组均增加（43.2±8.7）%，（17.9±6.6）%，（21.8±7.5）%（0.01）. 结论 脾虚证大鼠和患者胃粘膜组织中D细胞阳性和强阳性细胞率增加，提示脾虚患者临床出现腹胀、纳差等症状可能是由于SS内分泌细胞功能增强所致. 　　Keywords:spleen deficiencysomatostatinSijunzi decoc-tion， enteroendocrine cells immunohis-tochemistrymedicine，Chinese 　　Abstract:AIM To investigate the relationship between so-matostain enteroendocrine cells and spleen-asthenia syndrome in rats and patients.METHODS Short-term and the long-term rat models of spleen-asthenia syndrome were treated by reserpine for seven days and fourteen days.The patients with spleen-asthenia syndrome（12），stomach-heat syndrome（11）and flaming-up of liver-fire syndrome（9）were selected by differential diagnosis and immunohistochemical SABC method was employed to stain the D cells of gastric antrum.The variation of appearance and quantity of endocrine cells were observed by light microscope.RESULTS In the rat model of seven days，as compared with the controls，the number of D cells increased（43.4±3.1），（78.6±4.6）（0.01），and the percentage of strongly positive D cells reduced（34.6±2.5）%，（24.1±3.5）%（0.01）.In the rat model of fourteen days，the percentage of strongly positive D cells in-creased（34.6±2.5）%，（46.4±9.0）%（0.01），In pa-tients with spleen-asthenia syndrome the quantity（70.4±10.1），（38.1±6.4），（45.4±8.6）%and the percentage of strongly positive of D cell（43.2±8.7）%，（17.9±6.6）%，（21.8±7.5）%（0.01）increased as compared with the other groups.CONCLUSION Quantity and the percentage of strongly positive D cells