苦丁茶冬青叶多糖的提取及含量测定_药学论文.docVIP

苦丁茶冬青叶多糖的提取及含量测定_药学论文 苦丁茶冬青叶多糖的提取及含量测定_药学论文 【关键词】 苦丁茶冬青叶多糖；,,提取；,,苯酚 　　摘要：目的研究苦丁茶冬青叶多糖含量的测定方法。方法以水提醇沉的方法从苦丁茶冬青叶中提取得到苦丁茶冬青叶多糖并以正交实验优化显色条件，采用苯酚-硫酸法测定其多糖含量。结果测定波长为482 nm，最佳显色条件为6%苯酚用量1.0 ml，浓硫酸用量7.0 ml，反应时间40 min。标准曲线回归方程为A＝0.054 6C-0.003 3（n＝10），r＝0.999 8，并在2.51～45.11 mg#12539;L1范围内呈良好线性关系。平均回收率为98.55％，RSD＝1.86％（n＝5）。测得多糖平均含量为31.90％，RSD＝1.75％（n＝6）。结论该方法简单、准确、重现性好，可为其继续研究开发及应用提供参考依据。 　　关键词：苦丁茶冬青叶多糖； 提取； 苯酚硫酸法； 含量测定； 正交实验 　　Extraction and Determination of the Polysaccharides from the Leaves of Ilex kudincha C. J. Tseng 　　Abstract：ObjectiveTo study the determination method for polysaccharides in the leaves of Ilex kudincha C. J. Tseng.MethodsThe leaves of Ilex kudincha C. J. Tseng were defatted with 95% EtOH，extracted with water and precipitated with 80% EtOH, and then was deproteined three times by Sevag method, and from which, a crude polysaccharide noted as KPSⅡwas obtained. The traditional colorimetric method applied for the determination of polysaccharides from the leaves of Ilex kudincha C. J. Tseng was developed by the use of phenylhydrate-sulfuric acid, and was optimized by orthogonal experiment.Results The results showed that the wavelength for measurement was 482 nm. The best colorimetric conditions were the time of reaction of 40 min and the dosage of 6% phenylhydrate and H2SO4 of 1.0 and 7.0 ml, respectively. Linear range was 2.51～45.11 mg#12539;L1(A＝0.054 6C-0.003 3，r＝0.999 8). The average recovery was 98.55% and RSD was 1.86% (n＝5). The polysaccharide content of KPSⅡ was 31.90％ and RSD was 1.75％ (n＝6). ConclusionThe method used in this paper is simple, accurate and stable. The result obtained may act as a base for the further study and application of it. 　　Key words：Polysaccharides from the leaves of Ilex kudincha C. J. Tseng; Extraction； Phenylhydrate-sulfuric acid method； Determination； Orthogonal experiment 　　苦丁茶冬青Ilex kudincha C. J. Tseng是冬青科（Aquifoliaceae）冬青属乔木植物，主产于湖南、湖北、广东、广西、海南等省。叶（苦丁茶）苦、甘、凉。清热解毒，祛暑。主要用于治疗头痛、齿痛、目赤、热病烦渴、痢疾等症［1］。近年来，已有人对苦丁茶冬青叶（苦丁茶）