苦参素防治阿霉素肾病鼠肾小球硬化_医学论文.docVIP

苦参素防治阿霉素肾病鼠肾小球硬化_医学论文 苦参素防治阿霉素肾病鼠肾小球硬化_医学论文 【关键词】 苦参素；多柔比星/副作用；肾病综合症/化学诱导；大鼠；Smads蛋白；转化生长因子β1 　Experimental study of oxymatrine in preventing glomerulosclerosis in rats with adriamycininduced nephropathy 【Abstract】 AIM: To observe the longterm renoprotective effects of oxymatrine on the adriamycininduced nephropathy rats with chronic progressive renal lesions, and explore its mechanisms. METHODS: The experiments were performed on 60 male Wistar rats. The rats were randomly divided into 4 groups: normal control group, oxymatrine 50 mg/(kg#12539d) treatment group, oxymatrine 100 mg/(kg#12539d) treatment group, model group. Adriamycin (2 mg/kg) was intravenously administered twice at a 21day interval. Oxymatrine was used for gastric perfusion from the 1st day after the second injection of adriamycin until the end of the study. After 7 weeks, 5 rats in each group were sacrificed every 8 weeks for blood biochemical analyses and histological study. Urinary protein (mg/24 h), the concentrations of serum creatinine (Cr) and blood urea nitrogen (BUN) were checked with automatic biochemistry analyzer, and a semiquantitative score was used to evaluate the degree of glomerular lesions. Finally, immunohistochemistry was to examine the expression of transforming growth factorβ1 (TGFβ1), Smad1, 2, 3, 5 and Smad7 in glomeruli. RESULTS: Oxymatrine not only reduced urinary protein, Cr and BUN in blood, but also significantly ameliorated glomerular sclerosis and mesangial proliferation. Meanwhile, immunohistochemistry staining indicated that TGFβ1, Smad1, 2, 3, 5 expressions increased and Smad7 expression decreased in model group as compared with the 2 treatment groups. CONCLUSION: Oxymatrine has a renoprotective effect on the adriamycininduced nephropathy rats with chronic progressive renal lesions, and its mechanism is considered to be relevant to intervention of the pathway of signal conduction of TGFβ1/ Smads. 【Keywords】 oxymatrine doxorubicin/adverse effect nephrotic syndrome/