腺病毒介导KDR启动子的胸苷激酶基因治疗小鼠人鼻咽癌模型_医学论文.docVIP

腺病毒介导KDR启动子的胸苷激酶基因治疗小鼠人鼻咽癌模型_医学论文 腺病毒介导KDR启动子的胸苷激酶基因治疗小鼠人鼻咽癌模型_医学论文 【关键词】 鼻咽肿瘤 　　Adenovirusmediated herpes simplex virus thymidine kinase gene transfer under the driving of KDR promoter in treatment of experimental human nasopharyngeal carcinoma in nude mice 　　【Abstract】 AIM: To investigate the therapeutic efficacy of adenovirusmediated herpes simplex virus thymidine kinase (HSVtk) gene transfer under the driving of KDR promoter (AdKDRtk) in combination of ganciclovir(GCV) against human nasopharyngeal carcinoma(NPC) in nude mice. METHODS: CNE2 cell line was implanted subcutaneously into 36 nude mice, which were subsequently divided into 3 groups (n=12 each group): AdKDRtk/GCV group, AdCMVtk/GCV group (under the driving of CMV promoter) and saline/GCV group. Then the selective intratumoral injection of recombinant adenovirus or saline was performed in all nude mice，and repeated 24 h later. For the following 10 d GCV was given at a dose of 100 mg/（kg#12539d）, ip. All the treated animals were killed to evaluate the tumor weight and the histopathological changes and the microvessel density of tumors after the treatment finished. RESULTS: The tumor weight and microvessel density of the saline/GCV group, AdCMVtk/GCV group and AdKDRtk/GCV were （2.53±1.21）g and (17.78±2.12) /mm3, (1.81±0.84) g and (11.54±1.56) /mm3, (1.48±0.88) g and (9.87±2.18) /mm3 respectively. Growth and microvessel density of NPC was significantly inhibited in the AdCMVtk/GCV group and AdKDRtk/GCV group as compared with that in the saline/GCV group (0.05). AdKDRtk/GCV group showed more marked antitumor effect, compared with the AdCMVtk/GCV group (0.05). CONCLUSION: Intratumoral injection of AdKDRtk results in the marked inhibition of NPC growth in nude mice. It may be a new treatment approach for human NPC. 　　【Keywords】 nasopharyngeal neoplasms KDR promoter simplervirus thymidine kinase adenovirus vector 　　【摘要】 目的： 研究腺病毒介导的KDR启动子单纯疱疹病毒胸苷激酶(HSVtk)系统(AdKDR tk)结合丙氧鸟苷（GCV）对裸鼠人鼻咽癌的治疗作用. 方法： 建立人鼻咽癌裸鼠模型, 将36只