腺苷诱导急性冠脉综合征患者单核细胞血管内皮生长因子表达-_医学论文.docVIP

腺苷诱导急性冠脉综合征患者单核细胞血管内皮生长因子表达-_医学论文 腺苷诱导急性冠脉综合征患者单核细胞血管内皮生长因子表达*_医学论文 张 翼，杨玉莲，郭 莹，欧柏青，宁忠平，骆阳平，崔 波，唐铭强，付庆华 【摘要】 目的 探讨腺苷是否能诱导急性冠脉综合征（ACS）患者外周血单核细胞中血管内皮生长因子（VEGF）的表达。方法 分别抽取80例ACS患者发病后第1天的外周静脉血，随机分为4组，每组20例，对外周血单核细胞进行分离、培养，其中3组分别加入腺苷10μg、100μg、1000μg培养。用酶联免疫吸附法（ELISA）测定VEGF浓度。结果 ACS患者VEGF水平随着培养时间的延长而增加，在腺苷10μg组与对照组同时程比差异无显著性（P＞0.05）。VEGF水平在腺苷100μg组和1000μg组均显著高于同时程对照组（P＜0.05，P＜0.01），与腺苷浓度和作用时间成正比。结论 腺苷能诱导ACS患者外周血单核细胞中血管内皮生长因子表达且与浓度和作用时间成正比。 【关键词】 腺苷；急性冠脉综合征；外周血单核细胞；血管内皮生长因子 【Abstract】 Objective To investigate expression of vascular endothelial growth factor by peripheral blood mononuclear cells with adenosine in patients with acute coronary syndrome.Methods 80 patients with acute coronary syndrome(ACS) were used for this study.Peripheral blood mononuclear cells，(PBMCs) were isolated from peripheral blood on the 1st day after the onset of ACS.PBMCs were cultured with normal sodium (control group) and adenosine 10μg，100μg，1000μg for 6h，12h，24h.Vascular endothelial growth factor (VEGF) levels in the culture media were measured by enzyme-linked immunosorbent assay (ELISA).Results VEGF levels in the cultured medium of PBMCs after incubation of 6h，12h，24h these group were significantly higher (P＜0.05，P＜0.01)，but in adenosine 10μg group were insignificant.Conclusion This study showed that adenosine can induce the expression of vascular endothelial growth factor by peripheral blood mononuclear cells in patients with acute coronary syndrome.VEGF levels were increased gradually along with the action time and concentration of adenosine. 【Key words】 adenosine；acute coronary syndrome；peripheral blood mononuclear cells；vascular endothelial growth factor 目前应用血管生长基因来促进缺血心肌血管再生，即分子搭桥的方法在治疗急性冠脉综合征（acute coronary syndrome，ACS)方面正日益受到重视，血管内皮生长因子（vascular endothelial growth factor，VEGF）已被证实［1，2］能促血管生成，重建冠状动脉侧支循环形成，改善心功能。研究发现［3］急性心肌梗死（acute myocardial infarction，AMI）患者左室收缩功能改善与外周血单核细胞（peripheral blood mononuclear cells，PBMCs）源性VEGF呈正相关。腺苷（adenosine，Ado）被公认为能使缺血心肌［4］产生药理性预适应，对缺血心肌有保护作