血竭提取物对人成纤维细胞增殖的影响_医学论文.docVIP

血竭提取物对人成纤维细胞增殖的影响_医学论文 血竭提取物对人成纤维细胞增殖的影响_医学论文 作者：李丹，郭树忠，王胜春，张琳西，胡永武 【关键词】 血竭；成纤维细胞；细胞增殖；伤口愈合 　　Effect of Dragons blood extracts on proliferation of human fibroblast cells　　【Abstract】 AIM: To investigate the effect of Dragons blood on the proliferation of fibroblast cells in vitro and to explore the mechanism by which Dragons blood promotes wound healing. METHODS: Human fibroblast cells were cultured in vitro in media containing gradient concentrations of 3 kinds of Dragons blood extracts (extracted by chloroform, acetidin and alcohol, respectively), which was followed by cell proliferation determination assessed with MTT assay at different time intervals. The growth curve at the most suitable concentration was drawn. The flow cytometry (FCM) was used to determine the changes of cell cycle at this concentration. RESULTS: The 0.5-2.0 g/L dilution of Dragons blood extract by acetidin enhanced the proliferation of fibroblast in a dosedependent manner. The 2.0 g/L was the optimal concentration of Dragons blood extract, and it could increase the cell ratio in Sphase of cell cycle by 18.3% of that in control group (0.01). CONCLUSION: Dragons blood extract by acetidin could promote the proliferation of cultured human fibroblast cells in vitro, and it might be beneficial to granulation tissue formation during wound healing.　　【Keywords】 resina draconis fibroblasts cell proliferation wound healing 　　【摘要】 目的： 观察血竭提取物对体外培养成纤维细胞增殖的影响，探讨血竭促进创面愈合的机制. 方法： 将血竭经过氯仿、乙酸乙酯、乙醇回流提取得到的3种提取液，分别加入培养液并进行成纤维细胞的培养，MTT法检测不同血竭提取物在不同浓度以及不同时间点对体外培养成纤维细胞增殖的影响， 并绘制最适浓度条件下细胞生长曲线. 利用流式细胞仪分析最适浓度培养条件下成纤维细胞的细胞周期变化. 结果： 血竭乙酸乙酯提取物在0.5~2.0 g/L浓度范围内，促进成纤维细胞增殖，且呈剂量依赖性，在浓度为2.0 g/L时促进作用最为显著，此条件下处于S期的细胞较对照组增加18.3% (0.01). 结论： 血竭乙酸乙酯提取物能显著促进成纤维细胞增殖，可能与血竭促进创面愈合的机制有关.　　【关键词】 血竭；成纤维细胞；细胞增殖；伤口愈合 　　0引言 　　血竭（Dragons blood）在我国传统医学中的应用历史悠久，具有活血化瘀、祛腐生肌、收敛止血、消肿止痛等功效［1］. 目前临床应用血竭及含有血竭的生肌药物治疗难愈性创面，具有确切的疗效，但其作用机制尚不明确，以往的研究多在探讨血竭活血化瘀、改善创面血供方面的作用［2］，不能完全解释其促进创面愈合的作用机制. 成纤维细胞是创面修复过程中的最主要的功能细胞，在伤口愈合过程中经过迁移、增殖、分泌大量的胶原纤维和基质成份，与新生毛细血管