血管内照射对抑制兔血管损伤术后平滑肌细胞增殖疗效观察_医学论文.docVIP

血管内照射对抑制兔血管损伤术后平滑肌细胞增殖疗效观察_医学论文 血管内照射对抑制兔血管损伤术后平滑肌细胞增殖疗效观察_医学论文 作者:曹莉，曾知恒，刘唐威，黄凯，覃伟武 【摘要】 目的 观察188铼血管内照射对抑制新西兰白兔血管损伤术后平滑肌细胞增殖疗效。方法 40只新西兰白兔行腹主动脉球囊内皮拉伤后分别予188Re血管内照射（照射组），不予血管内照射（对照组），分析术后3、6周病理组织学标本平滑肌细胞增殖和凋亡情况。结果 照射组3周平滑肌细胞比对照组增殖细胞明显下降，凋亡细胞明显增多（P＜0.05），6周两组平滑肌细胞增殖、凋亡无显著性意义(P＞0.05)。结论 血管内照射能有效抑制新西兰白兔血管损伤术后平滑肌细胞增殖，促进平滑肌细胞细胞凋亡。 【关键词】 新西兰白兔；再狭窄；188铼；血管内照射 Effects of intravascular irradiation on inhibition proliferation of rabbit vessel smooth muscle cells after balloon angioplasty 【Abstract】 Objective To observe the effect of 188 Re intravascular irradiation on inhibiting proliferation of rabbit vessel smooth muscle cells(VSMCs) after balloon angioplasty. Methods Forty New Zealand white rabbits were divided into two groups to perform balloon catheter overstretched on abdominal aortic artery. In irradiation group，188Re-radioactive liquid filled balloon irradiation was performed in target segment. Proliferation and apoptosis of VSMCs in target segment were analysis after 3 and 6 weeks. Results In 3 weeks，proliferation cells were decreased significantly and apoptosis cells were increased significantly in irradiation group than that in control group (P＜0.05)，and there were no significant difference between two groups in 6 weeks(P＞0.05). Conclusion Applied 188 Re to intravascular irradiation can inhibit the proliferation and accelerate the apoptosis of VSMCs after balloon angioplasty efficiently. 【Key words】 New Zealand white rabbit；restenosis；188Re；intravascular irradiation 血管再狭窄(RS)是导致冠状动脉成形术(PTCA)失败的主要原因之一。PTCA术后机械损伤刺激了血管平滑肌细胞（VSMCs）增殖，使其表型由收缩型转换为合成型，迁移、增殖能力增强，促进细胞外基质合成，导致管腔RS的发生［1］。血管内照射治疗是抑制细胞增殖的有效方法，动物实验及临床研究表明［2，3］，血管内照射能抑制新生内膜增生，预防损伤血管RS。本实验采用β-放射性核素188Re对新西兰白兔血管损伤术后进行血管内照射，观察血管内照射对术后不同时点血管平滑肌细胞的增殖、凋亡的影响，旨在证实血管内照射治疗预防血管再狭窄的可行性及有效性。 1 材料与方法 1.1 动物分组 健康新西兰大白兔40只，雌雄各半，体重2.2～2.5kg，随机分为对照组和照射组，每组20只，再将两组随机分为2个亚组，每个亚组10只，普通兔颗粒饲料喂养，由广西医科大学动物实验中心提供。 1.2 实验仪器及试剂 (1)188钨/188铼(188Ｗ/188Re)发生器由上海新科药业股份公司提供，每次实验前用生理盐水从发生器中淋洗得到188Re，测定放射性浓度为1153.58～2132.31G