成膜法制作壳聚糖管状支架及支架的理化生物特性研究.pdfVIP

成膜法制作壳聚糖管状支架及支架的 理化生物特性研究 陈刚，刘汉云，张自正，饶新辉 (中山大学附属梅州市人民医院，广东梅州514031) 摘要：目的探讨成膜法自制壳聚糖管状支架的方法及支架的理化学生物特性。方法 利用乙酸溶液制备浓 度为8％的壳聚糖乙酸水溶胶，采用成膜方法制管后用NaOH脱下壳聚糖导管，扫描电镜下观察壳聚糖管表面超微 结构；OF进行溶胀性实验、pH值测定、细胞毒性试验、体外及体内降解实验。结果壳聚糖管光滑，韧性好，具有三 维多孔立体结构，孔径大小不同。壳聚糖管具有膨胀性，中性，无毒，随时间被组织吸收降解，体外降解慢于体内。 结论成膜法自制壳聚糖管状支架可行，其理化生物学特性表明可用作生物可吸收支架。 关键词：壳聚糖；生物高分子材料；导管支架；生物降解 doi：10．3969／j．issn．1002-266X．2013．38．003 中图分类号：R318 文献标志码：A 文章编号：1002-266X(2013)38-0008-03 Film methodforchitosantubularscaffoldsandthe and forming physicOchemicaI biologicalproperties Xin—hui CHEN能昭，LIUHan—yun，ZHANGZi—zheng，RAO (Meizhou toSunYat—sen 51403 PeoplesHospitalAffiliated University，Meizhou1，China) To thefilm for methodehitosantubularscaffolds andthe Abstract：Objectiveinvestigateforming making physico- chemicaland The8％chitosanaceticacid was aceticacid biologicalproperties．Methods hydrosolpreparedby solution． andformedthetube film weremovedthechitosantubeswithNaOHsolutionto tubularscaf- method，and by forming get electron usedtofurther thesurfaceultrastructureof theex- folds．Scanningmicroscopy(SEM)Wasexplore scaffolds．then about invitroandvivo made．Results perimentsswelling，PH，celltoxicity，degradationwere Thechitosantubularseaf- foldsweresmoothandwith structureanddifferentsize were goodtenacity，three-dimensionalporous pores；theyswelling， withthetime wereabsorbedand Wasslowerinvitro neutral，an